The 5′ and 3′ downstream AUG region elements are required for mosquito-borne flavivirus RNA replication

Peter Friebe, Pei Yong Shi, Eva Harris

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Flaviviruses require complementarity between the 5′ and 3′ ends of the genome for RNA replication. For mosquito-borne flaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3′ DAR sequence is also part of a hairpin (HP-3′SL), and both complementarity between 5′ and 3′ DAR motifs and formation of the HP-3′SL in the absence of the 5′ end are conserved among mosquito-borne flaviviruses. Using West Nile virus as a model, we demonstrate that 5′-3′ DAR complementarity and HP-3′SL formation are essential for viral RNA replication.

Original languageEnglish (US)
Pages (from-to)1900-1905
Number of pages6
JournalJournal of virology
Volume85
Issue number4
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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