The 5′ and 3′ downstream AUG region elements are required for mosquito-borne flavivirus RNA replication

Peter Friebe, Pei-Yong Shi, Eva Harris

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Flaviviruses require complementarity between the 5′ and 3′ ends of the genome for RNA replication. For mosquito-borne flaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3′ DAR sequence is also part of a hairpin (HP-3′SL), and both complementarity between 5′ and 3′ DAR motifs and formation of the HP-3′SL in the absence of the 5′ end are conserved among mosquito-borne flaviviruses. Using West Nile virus as a model, we demonstrate that 5′-3′ DAR complementarity and HP-3′SL formation are essential for viral RNA replication.

Original languageEnglish (US)
Pages (from-to)1900-1905
Number of pages6
JournalJournal of Virology
Volume85
Issue number4
DOIs
StatePublished - Feb 2011
Externally publishedYes

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Flaviviridae
Flavivirus
Culicidae
Viral RNA
RNA
West Nile virus
Dengue virus
Dengue Virus
Cyclization
Genome
genomics
genome
RNA replication

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The 5′ and 3′ downstream AUG region elements are required for mosquito-borne flavivirus RNA replication. / Friebe, Peter; Shi, Pei-Yong; Harris, Eva.

In: Journal of Virology, Vol. 85, No. 4, 02.2011, p. 1900-1905.

Research output: Contribution to journalArticle

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