The 5′-end sequence of the murine coronavirus genome

Implications for multiple fusion sites in leader-primed transcription

Chien Kou Shieh, Lisa H. Soe, Shinji Makino, Ming Fu Chang, Stephen A. Stohlman, Michael M C Lai

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The coronavirus leader-primed transcription model proposes that free leader RNA species derived from the 5′-end of the genomic RNA are utilized as a primer for the transcription of subgenomic mRNAs. To elucidate the precise mechanism of leader-priming, we cloned and sequenced the 5′-end of the mouse hepatitis virus genomic RNA. The 5′-terminal sequences are identical to the leader sequences present at the 5′-end of the subgenomic mRNAs. Two possible hairpin loop structures and an AU-rich region around the 3′-end of the leader sequence may provide the termination site for leader RNA synthesis. The comparison of 5′-end genomic sequences and the intergenic start sites for mRNA transcription revealed that there are homologous regions of 7-18 nucleotides at the putative leader/body junction sites. Some intergenic regions contain a mismatching nucleotide within this homologous region. We propose that free leader RNA binds to the intergenic region due to this homology and is cleaved at the mismatching nucleotide before serving as a primer. Thus, the free leader RNA species may be longer than the leader sequences in the subgenomic mRNAs and different mRNAs may have different leader/body junction sites.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalVirology
Volume156
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Coronavirus
Genome
RNA
Intergenic DNA
Messenger RNA
Nucleotides
Murine hepatitis virus
Transcription Initiation Site

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

The 5′-end sequence of the murine coronavirus genome : Implications for multiple fusion sites in leader-primed transcription. / Shieh, Chien Kou; Soe, Lisa H.; Makino, Shinji; Chang, Ming Fu; Stohlman, Stephen A.; Lai, Michael M C.

In: Virology, Vol. 156, No. 2, 1987, p. 321-330.

Research output: Contribution to journalArticle

Shieh, Chien Kou ; Soe, Lisa H. ; Makino, Shinji ; Chang, Ming Fu ; Stohlman, Stephen A. ; Lai, Michael M C. / The 5′-end sequence of the murine coronavirus genome : Implications for multiple fusion sites in leader-primed transcription. In: Virology. 1987 ; Vol. 156, No. 2. pp. 321-330.
@article{555fddabab624b228ea3ef5df63e2741,
title = "The 5′-end sequence of the murine coronavirus genome: Implications for multiple fusion sites in leader-primed transcription",
abstract = "The coronavirus leader-primed transcription model proposes that free leader RNA species derived from the 5′-end of the genomic RNA are utilized as a primer for the transcription of subgenomic mRNAs. To elucidate the precise mechanism of leader-priming, we cloned and sequenced the 5′-end of the mouse hepatitis virus genomic RNA. The 5′-terminal sequences are identical to the leader sequences present at the 5′-end of the subgenomic mRNAs. Two possible hairpin loop structures and an AU-rich region around the 3′-end of the leader sequence may provide the termination site for leader RNA synthesis. The comparison of 5′-end genomic sequences and the intergenic start sites for mRNA transcription revealed that there are homologous regions of 7-18 nucleotides at the putative leader/body junction sites. Some intergenic regions contain a mismatching nucleotide within this homologous region. We propose that free leader RNA binds to the intergenic region due to this homology and is cleaved at the mismatching nucleotide before serving as a primer. Thus, the free leader RNA species may be longer than the leader sequences in the subgenomic mRNAs and different mRNAs may have different leader/body junction sites.",
author = "Shieh, {Chien Kou} and Soe, {Lisa H.} and Shinji Makino and Chang, {Ming Fu} and Stohlman, {Stephen A.} and Lai, {Michael M C}",
year = "1987",
doi = "10.1016/0042-6822(87)90412-0",
language = "English (US)",
volume = "156",
pages = "321--330",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The 5′-end sequence of the murine coronavirus genome

T2 - Implications for multiple fusion sites in leader-primed transcription

AU - Shieh, Chien Kou

AU - Soe, Lisa H.

AU - Makino, Shinji

AU - Chang, Ming Fu

AU - Stohlman, Stephen A.

AU - Lai, Michael M C

PY - 1987

Y1 - 1987

N2 - The coronavirus leader-primed transcription model proposes that free leader RNA species derived from the 5′-end of the genomic RNA are utilized as a primer for the transcription of subgenomic mRNAs. To elucidate the precise mechanism of leader-priming, we cloned and sequenced the 5′-end of the mouse hepatitis virus genomic RNA. The 5′-terminal sequences are identical to the leader sequences present at the 5′-end of the subgenomic mRNAs. Two possible hairpin loop structures and an AU-rich region around the 3′-end of the leader sequence may provide the termination site for leader RNA synthesis. The comparison of 5′-end genomic sequences and the intergenic start sites for mRNA transcription revealed that there are homologous regions of 7-18 nucleotides at the putative leader/body junction sites. Some intergenic regions contain a mismatching nucleotide within this homologous region. We propose that free leader RNA binds to the intergenic region due to this homology and is cleaved at the mismatching nucleotide before serving as a primer. Thus, the free leader RNA species may be longer than the leader sequences in the subgenomic mRNAs and different mRNAs may have different leader/body junction sites.

AB - The coronavirus leader-primed transcription model proposes that free leader RNA species derived from the 5′-end of the genomic RNA are utilized as a primer for the transcription of subgenomic mRNAs. To elucidate the precise mechanism of leader-priming, we cloned and sequenced the 5′-end of the mouse hepatitis virus genomic RNA. The 5′-terminal sequences are identical to the leader sequences present at the 5′-end of the subgenomic mRNAs. Two possible hairpin loop structures and an AU-rich region around the 3′-end of the leader sequence may provide the termination site for leader RNA synthesis. The comparison of 5′-end genomic sequences and the intergenic start sites for mRNA transcription revealed that there are homologous regions of 7-18 nucleotides at the putative leader/body junction sites. Some intergenic regions contain a mismatching nucleotide within this homologous region. We propose that free leader RNA binds to the intergenic region due to this homology and is cleaved at the mismatching nucleotide before serving as a primer. Thus, the free leader RNA species may be longer than the leader sequences in the subgenomic mRNAs and different mRNAs may have different leader/body junction sites.

UR - http://www.scopus.com/inward/record.url?scp=0023127902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023127902&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(87)90412-0

DO - 10.1016/0042-6822(87)90412-0

M3 - Article

VL - 156

SP - 321

EP - 330

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -