The application of PCR to epidemiological study on spotted fever group rickettsiae

Jianzhi Zhang, M. Y. Fan, D. Z. Bi

Research output: Contribution to journalArticle

Abstract

It was the first time that a primer pairs derived from the 190KDa protein antigen gene of R. rickettsii were used to amplify SFGR DNA in ticks, tick ova, larva, tick faeces and rodent organs which were collected in Hebei, Heilongjiang, Hainan and Beijing. A 532bp fragment was respectively amplified from above samples. The results were partially in concordance with data obtained through rickettsiae isolation. It was suggested that PCR is a rapid, specific, sensitive and practical method for detection of SFGR in endemic foci.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalZhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
Volume16
Issue number1
StatePublished - Feb 1995
Externally publishedYes

Fingerprint

Rickettsia
Ticks
Epidemiologic Studies
Fever
Polymerase Chain Reaction
vpr Genes
Feces
Ovum
Larva
Rodentia
Antigens
DNA
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The application of PCR to epidemiological study on spotted fever group rickettsiae. / Zhang, Jianzhi; Fan, M. Y.; Bi, D. Z.

In: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, Vol. 16, No. 1, 02.1995, p. 25-28.

Research output: Contribution to journalArticle

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