The Architecture of Yeast DNA Polymerase ζ

Yacob Gómez-Llorente, Radhika Malik, Rinku Jain, Jayati Roy Choudhury, Robert E. Johnson, Louise Prakash, Satya Prakash, Iban Ubarretxena-Belandia, Aneel K. Aggarwal

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalCell Reports
Volume5
Issue number1
DOIs
StatePublished - Oct 17 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'The Architecture of Yeast DNA Polymerase ζ'. Together they form a unique fingerprint.

  • Cite this

    Gómez-Llorente, Y., Malik, R., Jain, R., Choudhury, J. R., Johnson, R. E., Prakash, L., Prakash, S., Ubarretxena-Belandia, I., & Aggarwal, A. K. (2013). The Architecture of Yeast DNA Polymerase ζ. Cell Reports, 5(1), 79-86. https://doi.org/10.1016/j.celrep.2013.08.046