Abstract
DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-86 |
| Number of pages | 8 |
| Journal | Cell Reports |
| Volume | 5 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 17 2013 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
The Architecture of Yeast DNA Polymerase ζ. / Gómez-Llorente, Yacob; Malik, Radhika; Jain, Rinku; Choudhury, JayatiRoy; Johnson, Robert E.; Prakash, Louise; Prakash, Satya; Ubarretxena-Belandia, Iban; Aggarwal, Aneel K.
In: Cell Reports, Vol. 5, No. 1, 17.10.2013, p. 79-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Architecture of Yeast DNA Polymerase ζ
AU - Gómez-Llorente, Yacob
AU - Malik, Radhika
AU - Jain, Rinku
AU - Choudhury, JayatiRoy
AU - Johnson, Robert E.
AU - Prakash, Louise
AU - Prakash, Satya
AU - Ubarretxena-Belandia, Iban
AU - Aggarwal, Aneel K.
PY - 2013/10/17
Y1 - 2013/10/17
N2 - DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass
AB - DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass
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U2 - 10.1016/j.celrep.2013.08.046
DO - 10.1016/j.celrep.2013.08.046
M3 - Article
VL - 5
SP - 79
EP - 86
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 1
ER -