The Architecture of Yeast DNA Polymerase ζ

Yacob Gómez-Llorente, Radhika Malik, Rinku Jain, JayatiRoy Choudhury, Robert E. Johnson, Louise Prakash, Satya Prakash, Iban Ubarretxena-Belandia, Aneel K. Aggarwal

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalCell Reports
Volume5
Issue number1
DOIs
StatePublished - Oct 17 2013

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Accessories
DNA-Directed DNA Polymerase
Yeast
Catalytic Domain
Yeasts
DNA Damage
Electron Microscopy
DNA
Genome
Electron microscopy
Enzymes
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gómez-Llorente, Y., Malik, R., Jain, R., Choudhury, J., Johnson, R. E., Prakash, L., ... Aggarwal, A. K. (2013). The Architecture of Yeast DNA Polymerase ζ. Cell Reports, 5(1), 79-86. https://doi.org/10.1016/j.celrep.2013.08.046

The Architecture of Yeast DNA Polymerase ζ. / Gómez-Llorente, Yacob; Malik, Radhika; Jain, Rinku; Choudhury, JayatiRoy; Johnson, Robert E.; Prakash, Louise; Prakash, Satya; Ubarretxena-Belandia, Iban; Aggarwal, Aneel K.

In: Cell Reports, Vol. 5, No. 1, 17.10.2013, p. 79-86.

Research output: Contribution to journalArticle

Gómez-Llorente, Y, Malik, R, Jain, R, Choudhury, J, Johnson, RE, Prakash, L, Prakash, S, Ubarretxena-Belandia, I & Aggarwal, AK 2013, 'The Architecture of Yeast DNA Polymerase ζ', Cell Reports, vol. 5, no. 1, pp. 79-86. https://doi.org/10.1016/j.celrep.2013.08.046
Gómez-Llorente Y, Malik R, Jain R, Choudhury J, Johnson RE, Prakash L et al. The Architecture of Yeast DNA Polymerase ζ. Cell Reports. 2013 Oct 17;5(1):79-86. https://doi.org/10.1016/j.celrep.2013.08.046
Gómez-Llorente, Yacob ; Malik, Radhika ; Jain, Rinku ; Choudhury, JayatiRoy ; Johnson, Robert E. ; Prakash, Louise ; Prakash, Satya ; Ubarretxena-Belandia, Iban ; Aggarwal, Aneel K. / The Architecture of Yeast DNA Polymerase ζ. In: Cell Reports. 2013 ; Vol. 5, No. 1. pp. 79-86.
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