The bacteriophage T4 DNA injection machine

Michael G. Rossmann, Vadim V. Mesyanzhinov, Fumio Arisaka, Petr Leiman

Research output: Contribution to journalReview article

123 Citations (Scopus)

Abstract

The tail of bacteriophage T4 consists of a contractile sheath surrounding a rigid tube and terminating in a multiprotein baseplate, to which the long and short tail fibers of the phage are attached. Upon binding of the fibers to their cell receptors, the baseplate undergoes a large conformational switch, which initiates sheath contraction and culminates in transfer of the phage DNA from the capsid into the host cell through the tail tube. The baseplate has a dome-shaped sixfold-symmetric structure, which is stabilized by a garland of six short tail fibers, running around the periphery of the dome. In the center of the dome, there is a membrane-puncturing device, containing three lysozyme domains, which disrupts the intermembrane peptidoglycan layer during infection.

Original languageEnglish (US)
Pages (from-to)171-180
Number of pages10
JournalCurrent Opinion in Structural Biology
Volume14
Issue number2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Bacteriophage T4
Bacteriophages
Injections
Peptidoglycan
Capsid
DNA
Muramidase
Equipment and Supplies
Membranes
Infection

Keywords

  • Bacteriophage
  • Cryo-EM
  • Electron cryomicroscopy
  • Electron microscopy
  • EM
  • Gene product
  • gp
  • OB fold
  • Oligonucleotide/oligosaccharide-binding fold
  • Phage
  • Phage T4 lysozyme
  • T4L

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

The bacteriophage T4 DNA injection machine. / Rossmann, Michael G.; Mesyanzhinov, Vadim V.; Arisaka, Fumio; Leiman, Petr.

In: Current Opinion in Structural Biology, Vol. 14, No. 2, 04.2004, p. 171-180.

Research output: Contribution to journalReview article

Rossmann, Michael G. ; Mesyanzhinov, Vadim V. ; Arisaka, Fumio ; Leiman, Petr. / The bacteriophage T4 DNA injection machine. In: Current Opinion in Structural Biology. 2004 ; Vol. 14, No. 2. pp. 171-180.
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