The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical

Gregg T. Nagle, Sherry D. Painter, James E. Blankenship, Jerome V.A. Choate, Alexander Kurosky

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Egg laying in the marine molluscan genus Aplysia is elicited by an egg-laying hormone (ELH) which induces ovulation and acts on central neurons to effect egg-laying behavior. ELH, isolated from the A. californica bag cells, and three ELH-related peptides, isolated from the A. californica atrial gland, have been chemically characterized, yet relatively little is known about homologous peptides in other Aplysia species. In these studies, the primary structure of A. brasiliana ELH was determined. Bag cell clusters were extracted in an acidic solution, and the peptides purified by sequential gel filtration and reversed-phase HPLC; ELH was identified by bioassay. Amino acid compositional and sequence analyses demonstrated that the neurohormone was a 36-residue peptide whose sequence was identical to that of A. californica ELH: NH2-Ile- Ser-Ile-Asn-Gln-Asp-Leu-Lys-Ala-Ile-Thr-Asp-Met-Leu-Leu-Thr-Glu-Gln-Ile-Arg-Glu-Arg-Gln-Arg-Tyr-Leu-Ala- Asp-Leu-Arg-Gln-Arg-Leu-Leu-Glu-Lys-COOH.

Original languageEnglish (US)
Pages (from-to)867-872
Number of pages6
JournalPeptides
Volume9
Issue number4
DOIs
StatePublished - Jan 1 1988

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Keywords

  • Amino acid sequence
  • Aplysia brasiliana
  • Bag cells
  • Egg-laying hormone
  • Molluscan neuropeptide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Nagle, G. T., Painter, S. D., Blankenship, J. E., Choate, J. V. A., & Kurosky, A. (1988). The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical. Peptides, 9(4), 867-872. https://doi.org/10.1016/0196-9781(88)90135-0