The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical

Gregg T. Nagle, Sherry D. Painter, James E. Blankenship, Jerome V A Choate, Alexander Kurosky

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Egg laying in the marine molluscan genus Aplysia is elicited by an egg-laying hormone (ELH) which induces ovulation and acts on central neurons to effect egg-laying behavior. ELH, isolated from the A. californica bag cells, and three ELH-related peptides, isolated from the A. californica atrial gland, have been chemically characterized, yet relatively little is known about homologous peptides in other Aplysia species. In these studies, the primary structure of A. brasiliana ELH was determined. Bag cell clusters were extracted in an acidic solution, and the peptides purified by sequential gel filtration and reversed-phase HPLC; ELH was identified by bioassay. Amino acid compositional and sequence analyses demonstrated that the neurohormone was a 36-residue peptide whose sequence was identical to that of A. californica ELH: NH2-Ile- Ser-Ile-Asn-Gln-Asp-Leu-Lys-Ala-Ile-Thr-Asp-Met-Leu-Leu-Thr-Glu-Gln-Ile-Arg-Glu-Arg-Gln-Arg-Tyr-Leu-Ala- Asp-Leu-Arg-Gln-Arg-Leu-Leu-Glu-Lys-COOH.

    Original languageEnglish (US)
    Pages (from-to)867-872
    Number of pages6
    JournalPeptides
    Volume9
    Issue number4
    DOIs
    StatePublished - 1988

    Fingerprint

    Aplysia
    Ovum
    Hormones
    Peptides
    Bioassay
    Neurons
    Neurotransmitter Agents
    Mollusca egg-laying hormone
    Peptide Hormones
    Protein Sequence Analysis
    Ovulation
    Gels
    Biological Assay
    Gel Chromatography
    Amino Acids
    High Pressure Liquid Chromatography

    Keywords

    • Amino acid sequence
    • Aplysia brasiliana
    • Bag cells
    • Egg-laying hormone
    • Molluscan neuropeptide

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Cellular and Molecular Neuroscience

    Cite this

    Nagle, G. T., Painter, S. D., Blankenship, J. E., Choate, J. V. A., & Kurosky, A. (1988). The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical. Peptides, 9(4), 867-872. https://doi.org/10.1016/0196-9781(88)90135-0

    The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical. / Nagle, Gregg T.; Painter, Sherry D.; Blankenship, James E.; Choate, Jerome V A; Kurosky, Alexander.

    In: Peptides, Vol. 9, No. 4, 1988, p. 867-872.

    Research output: Contribution to journalArticle

    Nagle, GT, Painter, SD, Blankenship, JE, Choate, JVA & Kurosky, A 1988, 'The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical', Peptides, vol. 9, no. 4, pp. 867-872. https://doi.org/10.1016/0196-9781(88)90135-0
    Nagle, Gregg T. ; Painter, Sherry D. ; Blankenship, James E. ; Choate, Jerome V A ; Kurosky, Alexander. / The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical. In: Peptides. 1988 ; Vol. 9, No. 4. pp. 867-872.
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    abstract = "Egg laying in the marine molluscan genus Aplysia is elicited by an egg-laying hormone (ELH) which induces ovulation and acts on central neurons to effect egg-laying behavior. ELH, isolated from the A. californica bag cells, and three ELH-related peptides, isolated from the A. californica atrial gland, have been chemically characterized, yet relatively little is known about homologous peptides in other Aplysia species. In these studies, the primary structure of A. brasiliana ELH was determined. Bag cell clusters were extracted in an acidic solution, and the peptides purified by sequential gel filtration and reversed-phase HPLC; ELH was identified by bioassay. Amino acid compositional and sequence analyses demonstrated that the neurohormone was a 36-residue peptide whose sequence was identical to that of A. californica ELH: NH2-Ile- Ser-Ile-Asn-Gln-Asp-Leu-Lys-Ala-Ile-Thr-Asp-Met-Leu-Leu-Thr-Glu-Gln-Ile-Arg-Glu-Arg-Gln-Arg-Tyr-Leu-Ala- Asp-Leu-Arg-Gln-Arg-Leu-Leu-Glu-Lys-COOH.",
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