The basis for diminished functional recovery after delayed peripheral nerve repair

Tessa Gordon, Neil Tyreman, Mukaila Raji

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

The postsurgical period during which neurons remain without target connections (chronic axotomy) and distal nerve stumps and target muscles are denervated (chronic denervation) deleteriously affects functional recovery. An autologous nerve graft and cross-suture paradigm in Sprague Dawley rats was used to systematically and independently control time of motoneuron axotomy, denervation of distal nerve sheaths, and muscle denervation to determine relative contributions of each factor to recovery failure. Tibial (TIB) nerve was cross-sutured to common peroneal (CP) nerve via a contralateral 15mmnerve autograft to reinnervate the tibialis anterior (TA) muscle immediately or after prolonging TIB axotomy, CP autograft denervation, or TA muscle denervation. Numbers of motoneurons that reinnervated TA muscle declined exponentially from 99±15 to asymptotic mean (±SE) values of 35±1, 41±10, and 13±5, respectively. Enlarged reinnervated motor units fully compensated for reduced motoneuron numbers after prolonged axotomy and autograft denervation, but the maximal threefold enlargement did not compensate for the severe loss of regenerating nerves through chronically denervated nerve stumps and for failure of reinnervated muscle fibers to recover from denervation atrophy. Muscle force, weight, and cross-sectional area declined. Our results demonstrate that chronic denervation of the distal stump plays a key role in reduced nerve regeneration, but the denervated muscle is also a contributing factor. That chronic Schwann cell denervation within the nerve autograft reduced regeneration less than after the denervation of both CP nerve stump and TA muscle, argues that chronic muscle denervation negatively impacts nerve regeneration.

Original languageEnglish (US)
Pages (from-to)5325-5334
Number of pages10
JournalJournal of Neuroscience
Volume31
Issue number14
DOIs
StatePublished - Apr 6 2011
Externally publishedYes

Fingerprint

Denervation
Peripheral Nerves
Axotomy
Muscles
Autografts
Muscle Denervation
Motor Neurons
Peroneal Nerve
Nerve Regeneration
Tibial Nerve
Schwann Cells
Sutures
Atrophy
Sprague Dawley Rats
Regeneration
Transplants
Neurons
Weights and Measures

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The basis for diminished functional recovery after delayed peripheral nerve repair. / Gordon, Tessa; Tyreman, Neil; Raji, Mukaila.

In: Journal of Neuroscience, Vol. 31, No. 14, 06.04.2011, p. 5325-5334.

Research output: Contribution to journalArticle

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