The binding of silica to proteins from plasma and lungs of rat

In vitro

Shivendra V. Singh, Ballabh Das, M Khan, Qamar Rahman, P. N. Viswanathan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Silica dissolving out from the slate dust was found to bind with plasma protein and purified bovine serum albumin. At 24 h of incubation at 37°C binding affinity of silica (μg of silica bound/mg of protein) with plasma protein and bovine serum albumin was found to be 0.59 and 0.44, respectively. By molecular exclusion chromatography using Sephadex G-200, silica binding protein of plasma was determined to be of mol. wt. around 67 000. Similar proteins having silica binding capacity (mol. wt. 70 000 and 85 000) were also found in rat lung but these proteins unlike their plasma counterpart were glycoprotein in nature. Polyacrylamide gel electrophoresis of plasma and protein rich lung fraction show that proteins upon binding with silica undergo mobility changes. Significance of the existence of silica binding protein in plasma and lung of rat in relation to silica toxicity is discussed.

Original languageEnglish (US)
Pages (from-to)155-164
Number of pages10
JournalChemico-Biological Interactions
Volume49
Issue number1-2
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Silicon Dioxide
Rats
Blood Proteins
Lung
Bovine Serum Albumin
Plasmas
Carrier Proteins
Slate
Proteins
In Vitro Techniques
Chromatography
Electrophoresis
Dust
Protein Binding
Gel Chromatography
Toxicity
Polyacrylamide Gel Electrophoresis
Glycoproteins

Keywords

  • Binding protein
  • Interaction
  • Silicates
  • Slate dust

ASJC Scopus subject areas

  • Toxicology

Cite this

The binding of silica to proteins from plasma and lungs of rat : In vitro. / Singh, Shivendra V.; Das, Ballabh; Khan, M; Rahman, Qamar; Viswanathan, P. N.

In: Chemico-Biological Interactions, Vol. 49, No. 1-2, 1984, p. 155-164.

Research output: Contribution to journalArticle

Singh, Shivendra V. ; Das, Ballabh ; Khan, M ; Rahman, Qamar ; Viswanathan, P. N. / The binding of silica to proteins from plasma and lungs of rat : In vitro. In: Chemico-Biological Interactions. 1984 ; Vol. 49, No. 1-2. pp. 155-164.
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