Abstract
Silica dissolving out from the slate dust was found to bind with plasma protein and purified bovine serum albumin. At 24 h of incubation at 37°C binding affinity of silica (μg of silica bound/mg of protein) with plasma protein and bovine serum albumin was found to be 0.59 and 0.44, respectively. By molecular exclusion chromatography using Sephadex G-200, silica binding protein of plasma was determined to be of mol. wt. around 67 000. Similar proteins having silica binding capacity (mol. wt. 70 000 and 85 000) were also found in rat lung but these proteins unlike their plasma counterpart were glycoprotein in nature. Polyacrylamide gel electrophoresis of plasma and protein rich lung fraction show that proteins upon binding with silica undergo mobility changes. Significance of the existence of silica binding protein in plasma and lung of rat in relation to silica toxicity is discussed.
Original language | English (US) |
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Pages (from-to) | 155-164 |
Number of pages | 10 |
Journal | Chemico-Biological Interactions |
Volume | 49 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 1984 |
Externally published | Yes |
Keywords
- Binding protein
- Interaction
- Silicates
- Slate dust
ASJC Scopus subject areas
- Toxicology