The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2

A putative function in macrophage infection

Marcel Marín-Villa, Diego A. Vargas-Inchaustegui, Suzana P. Chaves, Antonio J. Tempone, Juliana M F Dutra, Maurilio J. Soares, Tania Ueda-Nakamura, Sergio C F Mendonça, Bartira Rossi-Bergmann, Lynn Soong, Yara M. Traub-Csekö

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cysteine proteinases have been implicated in many aspects of protozoan parasite pathogenesis. These hydrolases are normally found as zymogens, and some classes in trypanosomatids possess a long C-terminal extension (CTE), for which no function has been assigned. In this paper we hypothesize that the CTE domain of Lpcys2, the abundant lysosomal cysteine proteinase of Leishmania pifanoi amastigotes, is involved in host cell infection. Confirming previous reports that this peptide is highly immunogenic in Trypanosoma cruzi, we detected antibodies against CTE in sera of leishmaniasis patients. We produced a polyclonal antibody specific to Lpcys2 CTE and determined that this antibody was capable of recognizing both L. pifanoi and Leishmania amazonensis cysteine proteinases. Using this antibody, we detected a predominant localization of Lpcys2 CTE in the lysosome and flagellar pocket of cultured axenic amastigotes of both parasite species; however, its location was shifted towards the surface of the parasites during macrophage infection. We examined the role of Lpcys2 CTE in macrophage infection and found a significant reduction in the percentage of infected cells when macrophages were infected with L. pifanoi and L. amazonensis in the presence of anti-CTE antibody. This study suggests a role for leishmanial cysteine proteinases CTE at early stages of infection.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume162
Issue number1
DOIs
StatePublished - Nov 2008

Fingerprint

Cysteine Proteases
Leishmania
Macrophages
Antibodies
Infection
Parasites
Enzyme Precursors
Leishmaniasis
Trypanosoma cruzi
Hydrolases
Lysosomes
Peptides
Serum

Keywords

  • C-terminal extension
  • Cysteine proteinase
  • Infection
  • Leishmania
  • Macrophage

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Marín-Villa, M., Vargas-Inchaustegui, D. A., Chaves, S. P., Tempone, A. J., Dutra, J. M. F., Soares, M. J., ... Traub-Csekö, Y. M. (2008). The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2: A putative function in macrophage infection. Molecular and Biochemical Parasitology, 162(1), 52-59. https://doi.org/10.1016/j.molbiopara.2008.07.003

The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2 : A putative function in macrophage infection. / Marín-Villa, Marcel; Vargas-Inchaustegui, Diego A.; Chaves, Suzana P.; Tempone, Antonio J.; Dutra, Juliana M F; Soares, Maurilio J.; Ueda-Nakamura, Tania; Mendonça, Sergio C F; Rossi-Bergmann, Bartira; Soong, Lynn; Traub-Csekö, Yara M.

In: Molecular and Biochemical Parasitology, Vol. 162, No. 1, 11.2008, p. 52-59.

Research output: Contribution to journalArticle

Marín-Villa, M, Vargas-Inchaustegui, DA, Chaves, SP, Tempone, AJ, Dutra, JMF, Soares, MJ, Ueda-Nakamura, T, Mendonça, SCF, Rossi-Bergmann, B, Soong, L & Traub-Csekö, YM 2008, 'The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2: A putative function in macrophage infection', Molecular and Biochemical Parasitology, vol. 162, no. 1, pp. 52-59. https://doi.org/10.1016/j.molbiopara.2008.07.003
Marín-Villa, Marcel ; Vargas-Inchaustegui, Diego A. ; Chaves, Suzana P. ; Tempone, Antonio J. ; Dutra, Juliana M F ; Soares, Maurilio J. ; Ueda-Nakamura, Tania ; Mendonça, Sergio C F ; Rossi-Bergmann, Bartira ; Soong, Lynn ; Traub-Csekö, Yara M. / The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2 : A putative function in macrophage infection. In: Molecular and Biochemical Parasitology. 2008 ; Vol. 162, No. 1. pp. 52-59.
@article{f8f595cb024c4de4a8b0fd5b5ac6a9b8,
title = "The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2: A putative function in macrophage infection",
abstract = "Cysteine proteinases have been implicated in many aspects of protozoan parasite pathogenesis. These hydrolases are normally found as zymogens, and some classes in trypanosomatids possess a long C-terminal extension (CTE), for which no function has been assigned. In this paper we hypothesize that the CTE domain of Lpcys2, the abundant lysosomal cysteine proteinase of Leishmania pifanoi amastigotes, is involved in host cell infection. Confirming previous reports that this peptide is highly immunogenic in Trypanosoma cruzi, we detected antibodies against CTE in sera of leishmaniasis patients. We produced a polyclonal antibody specific to Lpcys2 CTE and determined that this antibody was capable of recognizing both L. pifanoi and Leishmania amazonensis cysteine proteinases. Using this antibody, we detected a predominant localization of Lpcys2 CTE in the lysosome and flagellar pocket of cultured axenic amastigotes of both parasite species; however, its location was shifted towards the surface of the parasites during macrophage infection. We examined the role of Lpcys2 CTE in macrophage infection and found a significant reduction in the percentage of infected cells when macrophages were infected with L. pifanoi and L. amazonensis in the presence of anti-CTE antibody. This study suggests a role for leishmanial cysteine proteinases CTE at early stages of infection.",
keywords = "C-terminal extension, Cysteine proteinase, Infection, Leishmania, Macrophage",
author = "Marcel Mar{\'i}n-Villa and Vargas-Inchaustegui, {Diego A.} and Chaves, {Suzana P.} and Tempone, {Antonio J.} and Dutra, {Juliana M F} and Soares, {Maurilio J.} and Tania Ueda-Nakamura and Mendon{\cc}a, {Sergio C F} and Bartira Rossi-Bergmann and Lynn Soong and Traub-Csek{\"o}, {Yara M.}",
year = "2008",
month = "11",
doi = "10.1016/j.molbiopara.2008.07.003",
language = "English (US)",
volume = "162",
pages = "52--59",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - The C-terminal extension of Leishmania pifanoi amastigote-specific cysteine proteinase Lpcys2

T2 - A putative function in macrophage infection

AU - Marín-Villa, Marcel

AU - Vargas-Inchaustegui, Diego A.

AU - Chaves, Suzana P.

AU - Tempone, Antonio J.

AU - Dutra, Juliana M F

AU - Soares, Maurilio J.

AU - Ueda-Nakamura, Tania

AU - Mendonça, Sergio C F

AU - Rossi-Bergmann, Bartira

AU - Soong, Lynn

AU - Traub-Csekö, Yara M.

PY - 2008/11

Y1 - 2008/11

N2 - Cysteine proteinases have been implicated in many aspects of protozoan parasite pathogenesis. These hydrolases are normally found as zymogens, and some classes in trypanosomatids possess a long C-terminal extension (CTE), for which no function has been assigned. In this paper we hypothesize that the CTE domain of Lpcys2, the abundant lysosomal cysteine proteinase of Leishmania pifanoi amastigotes, is involved in host cell infection. Confirming previous reports that this peptide is highly immunogenic in Trypanosoma cruzi, we detected antibodies against CTE in sera of leishmaniasis patients. We produced a polyclonal antibody specific to Lpcys2 CTE and determined that this antibody was capable of recognizing both L. pifanoi and Leishmania amazonensis cysteine proteinases. Using this antibody, we detected a predominant localization of Lpcys2 CTE in the lysosome and flagellar pocket of cultured axenic amastigotes of both parasite species; however, its location was shifted towards the surface of the parasites during macrophage infection. We examined the role of Lpcys2 CTE in macrophage infection and found a significant reduction in the percentage of infected cells when macrophages were infected with L. pifanoi and L. amazonensis in the presence of anti-CTE antibody. This study suggests a role for leishmanial cysteine proteinases CTE at early stages of infection.

AB - Cysteine proteinases have been implicated in many aspects of protozoan parasite pathogenesis. These hydrolases are normally found as zymogens, and some classes in trypanosomatids possess a long C-terminal extension (CTE), for which no function has been assigned. In this paper we hypothesize that the CTE domain of Lpcys2, the abundant lysosomal cysteine proteinase of Leishmania pifanoi amastigotes, is involved in host cell infection. Confirming previous reports that this peptide is highly immunogenic in Trypanosoma cruzi, we detected antibodies against CTE in sera of leishmaniasis patients. We produced a polyclonal antibody specific to Lpcys2 CTE and determined that this antibody was capable of recognizing both L. pifanoi and Leishmania amazonensis cysteine proteinases. Using this antibody, we detected a predominant localization of Lpcys2 CTE in the lysosome and flagellar pocket of cultured axenic amastigotes of both parasite species; however, its location was shifted towards the surface of the parasites during macrophage infection. We examined the role of Lpcys2 CTE in macrophage infection and found a significant reduction in the percentage of infected cells when macrophages were infected with L. pifanoi and L. amazonensis in the presence of anti-CTE antibody. This study suggests a role for leishmanial cysteine proteinases CTE at early stages of infection.

KW - C-terminal extension

KW - Cysteine proteinase

KW - Infection

KW - Leishmania

KW - Macrophage

UR - http://www.scopus.com/inward/record.url?scp=52049088493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52049088493&partnerID=8YFLogxK

U2 - 10.1016/j.molbiopara.2008.07.003

DO - 10.1016/j.molbiopara.2008.07.003

M3 - Article

VL - 162

SP - 52

EP - 59

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 1

ER -