The C-terminal helical domain of dengue virus precursor membrane protein is involved in virus assembly and entry

Szu Chia Hsieh, Gang Zou, Wen Yang Tsai, Min Qing, Gwong Jen Chang, Pei-Yong Shi, Wei Kung Wang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The role of the α-helical domain (MH) of dengue virus (DENV) precursor membrane protein in replication was investigated by site-directed mutagenesis. Proline substitutions of three residues (120, 123 and 127) at the C-terminus, but not those at the N-terminus of MH domain, reduced the virus-like particles of DENV1, DENV2 and DENV4 detected in supernatants. In a DENV2 replicon trans-packaging system, these three mutations suppressed particles detected; two of them (I123P and V127P) also affected viral entry. In the context of DENV2 genome-length RNA, all three mutations reduced virion assembly and virus spreading in cell culture. Analysis of revertants showed that mutation A120P could partially support viral infection cycle; in contrast, mutations I123P and V127P were lethal, and adaptations of I123P → I123L and V127P → V127L were required to restore the viral infection cycle. These findings demonstrate that the C-terminus of the MH domain is involved in both assembly and entry of DENV.

Original languageEnglish (US)
Pages (from-to)170-180
Number of pages11
JournalVirology
Volume410
Issue number1
DOIs
StatePublished - Feb 5 2011
Externally publishedYes

Fingerprint

Virus Assembly
Virus Internalization
Dengue Virus
Membrane Proteins
Mutation
Virus Diseases
Virion
Replicon
Product Packaging
Site-Directed Mutagenesis
Proline
Cell Culture Techniques
Genome
RNA

Keywords

  • Assembly
  • Dengue virus
  • Entry
  • Precursor membrane
  • Virus-like particles

ASJC Scopus subject areas

  • Virology

Cite this

The C-terminal helical domain of dengue virus precursor membrane protein is involved in virus assembly and entry. / Hsieh, Szu Chia; Zou, Gang; Tsai, Wen Yang; Qing, Min; Chang, Gwong Jen; Shi, Pei-Yong; Wang, Wei Kung.

In: Virology, Vol. 410, No. 1, 05.02.2011, p. 170-180.

Research output: Contribution to journalArticle

Hsieh, Szu Chia ; Zou, Gang ; Tsai, Wen Yang ; Qing, Min ; Chang, Gwong Jen ; Shi, Pei-Yong ; Wang, Wei Kung. / The C-terminal helical domain of dengue virus precursor membrane protein is involved in virus assembly and entry. In: Virology. 2011 ; Vol. 410, No. 1. pp. 170-180.
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