The calcium ionophore A23187 stimulates glycoprotein secretion by the guinea pig gallbladder

P. F. Malet, C. L. Locke, B. W. Trotman, R. D. Soloway

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to examine the role of calcium ions in gallbladder glycoprotein secretion in cultured guinea pig gallbladder explants. The calcium ionophore A23187 showed a threshold of 2 μg per ml medium for stimulation of secretion of [3H]glucosamine-labeled glycoproteins over a 30 min incubation period. The ionophore at 3 and 5 μg per ml medium resulted in a 3- to 4-fold increase in secretion of [3H]glucosamine-labeled glycoproteins. Ionophore-induced stimulation of glycoprotein secretion was abolished by the addition of 0.01 mM verapamil to the medium. To study the effect of changes in extracellular calcium on basal glycoprotein secretion, explants were cultured for 24 hr in media with 0.007, 0.5 or 2.0 mM calcium; no differences in basal glycoprotein secretion were observed. When cultured in medium with 1.0 mM EGTA, basal secretion decreased significantly vs. controls in 0.007 mM total calcium medium. Total [3H]glucosamine incorporation by explants in medium with EGTA was unaltered, however, suggesting that the low level of calcium in the medium was selectively impairing the secretory process. These findings indicate that calcium ions are important in the regulation of gallbladder glycoprotein secretion.

Original languageEnglish (US)
Pages (from-to)569-573
Number of pages5
JournalHepatology
Volume6
Issue number4
StatePublished - 1986
Externally publishedYes

Fingerprint

Calcium Ionophores
Calcimycin
Gallbladder
Glycoproteins
Guinea Pigs
Calcium
Glucosamine
Egtazic Acid
Ionophores
Ions
Secretory Pathway
Verapamil

ASJC Scopus subject areas

  • Hepatology

Cite this

Malet, P. F., Locke, C. L., Trotman, B. W., & Soloway, R. D. (1986). The calcium ionophore A23187 stimulates glycoprotein secretion by the guinea pig gallbladder. Hepatology, 6(4), 569-573.

The calcium ionophore A23187 stimulates glycoprotein secretion by the guinea pig gallbladder. / Malet, P. F.; Locke, C. L.; Trotman, B. W.; Soloway, R. D.

In: Hepatology, Vol. 6, No. 4, 1986, p. 569-573.

Research output: Contribution to journalArticle

Malet, PF, Locke, CL, Trotman, BW & Soloway, RD 1986, 'The calcium ionophore A23187 stimulates glycoprotein secretion by the guinea pig gallbladder', Hepatology, vol. 6, no. 4, pp. 569-573.
Malet, P. F. ; Locke, C. L. ; Trotman, B. W. ; Soloway, R. D. / The calcium ionophore A23187 stimulates glycoprotein secretion by the guinea pig gallbladder. In: Hepatology. 1986 ; Vol. 6, No. 4. pp. 569-573.
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