The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function

Laura A. Lindsey-Boltz, Geetanjali Chawla, N. Srinivasan, Usha Vijayraghavan, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, Prp17p is required for the efficient completion of the second step of pre-mRNA splicing. The function and interacting factors for this protein have not been elucidated. We have performed a mutational analysis of yPrp17p to identify protein domains critical for function. A series of deletions were made throughout the region spanning the N-terminal 158 amino acids of the protein, which do not contain any identified structural motifs. The C-terminal portion (amino acids 160-455) contains a WD domain containing seven WD repeats. We determined that a minimal functional Prp17p consists of the WD domain and 40 amino acids N-terminal to it. We generated a three-dimensional model of the WD repeats in Prp17p based on the crystal structure of the β-transducin WD domain. This model was used to identify potentially important amino acids for in vivo functional characterization. Through analysis of mutations in four different loops of Prp17p that lie between β strands in the WD repeats, we have identified four amino acids, 235TETG238, that are critical for function. These amino acids are predicted to be surface exposed and may be involved in interactions that are important for splicing. Temperature-sensitive prp17 alleles with mutations of these four amino acids are defective for the second step of splicing and are synthetically lethal with a U5 snRNA loop I mutation, which is also required for the second step of splicing. These data reinforce the functional significance of this region within the WD domain of Prp17p in the second step of splicing.

Original languageEnglish (US)
Pages (from-to)1289-1305
Number of pages17
JournalRNA
Volume6
Issue number9
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

RNA Precursors
Amino Acids
Mutation
Transducin
RNA Splicing Factors
Saccharomyces cerevisiae
Proteins
Alleles
Temperature
WD40 Repeats

Keywords

  • β propeller
  • Homology modeling
  • Mutational analysis
  • Nuclear localization
  • Prp17
  • Second-step splicing
  • U5 snRNA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. / Lindsey-Boltz, Laura A.; Chawla, Geetanjali; Srinivasan, N.; Vijayraghavan, Usha; Garcia-Blanco, Mariano.

In: RNA, Vol. 6, No. 9, 2000, p. 1289-1305.

Research output: Contribution to journalArticle

Lindsey-Boltz, Laura A. ; Chawla, Geetanjali ; Srinivasan, N. ; Vijayraghavan, Usha ; Garcia-Blanco, Mariano. / The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. In: RNA. 2000 ; Vol. 6, No. 9. pp. 1289-1305.
@article{e6fbd50450cd475090290519a5b063d8,
title = "The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function",
abstract = "In Saccharomyces cerevisiae, Prp17p is required for the efficient completion of the second step of pre-mRNA splicing. The function and interacting factors for this protein have not been elucidated. We have performed a mutational analysis of yPrp17p to identify protein domains critical for function. A series of deletions were made throughout the region spanning the N-terminal 158 amino acids of the protein, which do not contain any identified structural motifs. The C-terminal portion (amino acids 160-455) contains a WD domain containing seven WD repeats. We determined that a minimal functional Prp17p consists of the WD domain and 40 amino acids N-terminal to it. We generated a three-dimensional model of the WD repeats in Prp17p based on the crystal structure of the β-transducin WD domain. This model was used to identify potentially important amino acids for in vivo functional characterization. Through analysis of mutations in four different loops of Prp17p that lie between β strands in the WD repeats, we have identified four amino acids, 235TETG238, that are critical for function. These amino acids are predicted to be surface exposed and may be involved in interactions that are important for splicing. Temperature-sensitive prp17 alleles with mutations of these four amino acids are defective for the second step of splicing and are synthetically lethal with a U5 snRNA loop I mutation, which is also required for the second step of splicing. These data reinforce the functional significance of this region within the WD domain of Prp17p in the second step of splicing.",
keywords = "β propeller, Homology modeling, Mutational analysis, Nuclear localization, Prp17, Second-step splicing, U5 snRNA",
author = "Lindsey-Boltz, {Laura A.} and Geetanjali Chawla and N. Srinivasan and Usha Vijayraghavan and Mariano Garcia-Blanco",
year = "2000",
doi = "10.1017/S1355838200000327",
language = "English (US)",
volume = "6",
pages = "1289--1305",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "9",

}

TY - JOUR

T1 - The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function

AU - Lindsey-Boltz, Laura A.

AU - Chawla, Geetanjali

AU - Srinivasan, N.

AU - Vijayraghavan, Usha

AU - Garcia-Blanco, Mariano

PY - 2000

Y1 - 2000

N2 - In Saccharomyces cerevisiae, Prp17p is required for the efficient completion of the second step of pre-mRNA splicing. The function and interacting factors for this protein have not been elucidated. We have performed a mutational analysis of yPrp17p to identify protein domains critical for function. A series of deletions were made throughout the region spanning the N-terminal 158 amino acids of the protein, which do not contain any identified structural motifs. The C-terminal portion (amino acids 160-455) contains a WD domain containing seven WD repeats. We determined that a minimal functional Prp17p consists of the WD domain and 40 amino acids N-terminal to it. We generated a three-dimensional model of the WD repeats in Prp17p based on the crystal structure of the β-transducin WD domain. This model was used to identify potentially important amino acids for in vivo functional characterization. Through analysis of mutations in four different loops of Prp17p that lie between β strands in the WD repeats, we have identified four amino acids, 235TETG238, that are critical for function. These amino acids are predicted to be surface exposed and may be involved in interactions that are important for splicing. Temperature-sensitive prp17 alleles with mutations of these four amino acids are defective for the second step of splicing and are synthetically lethal with a U5 snRNA loop I mutation, which is also required for the second step of splicing. These data reinforce the functional significance of this region within the WD domain of Prp17p in the second step of splicing.

AB - In Saccharomyces cerevisiae, Prp17p is required for the efficient completion of the second step of pre-mRNA splicing. The function and interacting factors for this protein have not been elucidated. We have performed a mutational analysis of yPrp17p to identify protein domains critical for function. A series of deletions were made throughout the region spanning the N-terminal 158 amino acids of the protein, which do not contain any identified structural motifs. The C-terminal portion (amino acids 160-455) contains a WD domain containing seven WD repeats. We determined that a minimal functional Prp17p consists of the WD domain and 40 amino acids N-terminal to it. We generated a three-dimensional model of the WD repeats in Prp17p based on the crystal structure of the β-transducin WD domain. This model was used to identify potentially important amino acids for in vivo functional characterization. Through analysis of mutations in four different loops of Prp17p that lie between β strands in the WD repeats, we have identified four amino acids, 235TETG238, that are critical for function. These amino acids are predicted to be surface exposed and may be involved in interactions that are important for splicing. Temperature-sensitive prp17 alleles with mutations of these four amino acids are defective for the second step of splicing and are synthetically lethal with a U5 snRNA loop I mutation, which is also required for the second step of splicing. These data reinforce the functional significance of this region within the WD domain of Prp17p in the second step of splicing.

KW - β propeller

KW - Homology modeling

KW - Mutational analysis

KW - Nuclear localization

KW - Prp17

KW - Second-step splicing

KW - U5 snRNA

UR - http://www.scopus.com/inward/record.url?scp=0033827195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033827195&partnerID=8YFLogxK

U2 - 10.1017/S1355838200000327

DO - 10.1017/S1355838200000327

M3 - Article

VL - 6

SP - 1289

EP - 1305

JO - RNA

JF - RNA

SN - 1355-8382

IS - 9

ER -