The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit

Shamsher S. Saini, Erdem Tüzün, Premkumar Christadoss

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-α subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.

Original languageEnglish (US)
Pages (from-to)177-179
Number of pages3
JournalJournal of Neuroimmunology
Volume169
Issue number1-2
DOIs
StatePublished - Dec 2005

Fingerprint

Cholinergic Receptors
Protein Isoforms
Skeletal Muscle
Complementary DNA
Messenger RNA
Extremities
Cell Line
Muscles
Agar Gel Electrophoresis
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcription
Polymerase Chain Reaction

Keywords

  • Acetylcholine receptor alpha subunit
  • Autoimmunity
  • cDNA
  • Human rabdomyosarcoma cell line TE671
  • Myasthenia gravis
  • Ocular muscle
  • P3A exon
  • RT-PCR
  • Skeletal muscle

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit. / Saini, Shamsher S.; Tüzün, Erdem; Christadoss, Premkumar.

In: Journal of Neuroimmunology, Vol. 169, No. 1-2, 12.2005, p. 177-179.

Research output: Contribution to journalArticle

Saini, Shamsher S. ; Tüzün, Erdem ; Christadoss, Premkumar. / The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit. In: Journal of Neuroimmunology. 2005 ; Vol. 169, No. 1-2. pp. 177-179.
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