The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit

Shamsher S. Saini, Erdem Tüzün, Premkumar Christadoss

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-α subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.

Original languageEnglish (US)
Pages (from-to)177-179
Number of pages3
JournalJournal of Neuroimmunology
Volume169
Issue number1-2
DOIs
StatePublished - Dec 1 2005

Keywords

  • Acetylcholine receptor alpha subunit
  • Autoimmunity
  • Human rabdomyosarcoma cell line TE671
  • Myasthenia gravis
  • Ocular muscle
  • P3A exon
  • RT-PCR
  • Skeletal muscle
  • cDNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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