The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit

Shamsher S. Saini; Erdem Tüzün; Premkumar Christadoss

doi:10.1016/j.jneuroim.2005.08.013

The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit

Shamsher S. Saini
, Erdem Tüzün
, Premkumar Christadoss

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-α subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.

Original language	English (US)
Pages (from-to)	177-179
Number of pages	3
Journal	Journal of Neuroimmunology
Volume	169
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2005.08.013
State	Published - Dec 2005
Externally published	Yes

Keywords

Acetylcholine receptor alpha subunit
Autoimmunity
Human rabdomyosarcoma cell line TE671
Myasthenia gravis
Ocular muscle
P3A exon
RT-PCR
Skeletal muscle
cDNA

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2005.08.013

Cite this

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title = "The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit",

abstract = "The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-α subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.",

keywords = "Acetylcholine receptor alpha subunit, Autoimmunity, Human rabdomyosarcoma cell line TE671, Myasthenia gravis, Ocular muscle, P3A exon, RT-PCR, Skeletal muscle, cDNA",

author = "Saini, \{Shamsher S.\} and Erdem T{\"u}z{\"u}n and Premkumar Christadoss",

note = "Funding Information: This study is supported by AFM and Muscular Dystrophy Association. Authors thank Dr. Huan Yang for dissecting mouse ocular and limb muscles.",

year = "2005",

month = dec,

doi = "10.1016/j.jneuroim.2005.08.013",

language = "English (US)",

volume = "169",

pages = "177--179",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

number = "1-2",

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TY - JOUR

T1 - The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit

AU - Saini, Shamsher S.

AU - Tüzün, Erdem

AU - Christadoss, Premkumar

N1 - Funding Information: This study is supported by AFM and Muscular Dystrophy Association. Authors thank Dr. Huan Yang for dissecting mouse ocular and limb muscles.

PY - 2005/12

Y1 - 2005/12

N2 - The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-α subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.

AB - The mRNA isolated from mouse ocular and limb muscle and human rabdomyosarcoma cell line TE671 was subjected to reverse transcriptase polymerase chain reaction (RT-PCR) using a specific primer pair for the extracellular domain of AChR-α subunit that transcribes both for P3A- (isoform 1) and P3A+ (isoform 2). The cDNA synthesized from mRNA by reverse transcription, transcribed both isoforms 1 and 2 from mRNA of mouse limb and ocular muscle and human rabdomyosarcoma cell line TE671 as evidenced by agarose gel electrophoresis of polymerase chain reaction products.

KW - Acetylcholine receptor alpha subunit

KW - Autoimmunity

KW - Human rabdomyosarcoma cell line TE671

KW - Myasthenia gravis

KW - Ocular muscle

KW - P3A exon

KW - RT-PCR

KW - Skeletal muscle

KW - cDNA

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UR - https://www.scopus.com/pages/publications/27344459274#tab=citedBy

U2 - 10.1016/j.jneuroim.2005.08.013

DO - 10.1016/j.jneuroim.2005.08.013

M3 - Article

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AN - SCOPUS:27344459274

SN - 0165-5728

VL - 169

SP - 177

EP - 179

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

The cDNA of mouse skeletal muscle transcribe for both isoforms 1 and 2 of acetylcholine receptor alpha subunit

Abstract

Keywords

ASJC Scopus subject areas

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