The central spike complex of bacteriophage T4 contacts PpiD in the periplasm of Escherichia coli

Sabrina Wenzel, Mikhail M. Shneider, Petr G. Leiman, Andreas Kuhn, Dorothee Kiefer

Research output: Contribution to journalArticlepeer-review

Abstract

Infecting bacteriophage T4 uses a contractile tail structure to breach the envelope of the Escherichia coli host cell. During contraction, the tail tube headed with the “central spike complex” is thought to mechanically puncture the outer membrane. We show here that a purified tip fragment of the central spike complex interacts with periplasmic chaperone PpiD, which is anchored to the cytoplasmic membrane. PpiD may be involved in the penetration of the inner membrane by the T4 injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell. Host cells with the ppiD gene deleted showed partial reduction in the plating efficiency of T4, suggesting a supporting role of PpiD to improve the efficiency of the infection process.

Original languageEnglish (US)
Article number1135
JournalViruses
Volume12
Issue number10
DOIs
StatePublished - Oct 2020

Keywords

  • Bacteriophage T4
  • DNA translocation
  • Periplasmic chaperone
  • Tail structure

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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