The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin

Fei Gu, Tiffany S. Sullivan, Zhiwei Che, Chandrashekar Ganesa, William H. Flurkey, Robert F. Bozarth, Thomas Smith

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

KP4 is a virally encoded and highly specific toxin that kills fungi closely related to the fungus Ustilago maydis. The toxin was purified and crystals were formed using ammonium sulfate as precipitant. The crystals belong to the space group P61(5)22 and diffracted to ∼2.2 Å resolution. Circular dicroism spectroscopy suggests that the protein is predominantly comprised of β-strands.

Original languageEnglish (US)
Pages (from-to)792-795
Number of pages4
JournalJournal of Molecular Biology
Volume243
Issue number4
DOIs
StatePublished - Nov 4 1994
Externally publishedYes

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Keywords

  • crystallization
  • fungus
  • toxin
  • Ustilago maydis
  • virus

ASJC Scopus subject areas

  • Virology

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