The combination of growth hormone with hepatocyte growth factor alters the acute phase response

Marc G. Jeschke, Steven Wolf, Meelie A. DebRoy, David Herndon

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recombinant human growth hormone (rhGH) and hepatocyte growth factor (HGF) have both been shown to individually modulate hepatic acute phase reactant proteins and cytokine expression following trauma through different pathways. Recombinant hGH has also been shown to decrease serum and hepatic HGF concentrations after a thermal injury. We hypothesized that the combination of rhGH plus HGF improves the burn-induced acute phase response. Fifty-six male Sprague-Dawley rats received a 60% TBSA third-degree scald burn and were randomly divided to receive either rhGH (2.5 mg/kg/day sc.) plus HGF (200 μg/kg i.v. every 12 h) or placebo (saline). Rats were sacrificed on post-burn days 1, 2, 5, or 7 and serum constitutive and acute phase proteins, TNF-α, IL-1β, IL-6 and liver total protein measured. Hepatic cytokine gene expression, triglyceride concentration, and hepatocyte proliferation were also measured. In rats receiving rhGH/HGF, serum albumin increased on days 5 and 7 and transferrin on day 7 after burn compared to placebo (P < 0.05). Haptoglobin decreased 5 days after burn compared to placebo (P < 0.05). RhGH/HGF increased serum TNF-α on day 2 after burn, while it decreased serum IL-1β on day 1 after burn compared with placebo (P < 0.05). RhGH/HGF had no effect on hepatic cytokine gene expression compared with placebo. Liver total protein content and hepatocyte proliferation increased on days 1, 2, 5, and 7 after burn with rhGH/HGF treatment (P < 0.05). These findings indicate that rhGH in combination with HGF exert additive effects on constitutive hepatic proteins and partial inhibitory effects on acute phase protein and cytokine expression. RhGH/HGF has a strong mitogenic effect on hepatocytes.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalShock
Volume12
Issue number3
StatePublished - Sep 1999

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Acute-Phase Reaction
Hepatocyte Growth Factor
Growth Hormone
Human Growth Hormone
Acute-Phase Proteins
Liver
Placebos
Cytokines
Hepatocytes
Serum
Interleukin-1
Burns
Gene Expression
Proteins
Haptoglobins
Wounds and Injuries
Transferrin
Serum Albumin
Sprague Dawley Rats
Interleukin-6

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

The combination of growth hormone with hepatocyte growth factor alters the acute phase response. / Jeschke, Marc G.; Wolf, Steven; DebRoy, Meelie A.; Herndon, David.

In: Shock, Vol. 12, No. 3, 09.1999, p. 181-187.

Research output: Contribution to journalArticle

Jeschke, MG, Wolf, S, DebRoy, MA & Herndon, D 1999, 'The combination of growth hormone with hepatocyte growth factor alters the acute phase response', Shock, vol. 12, no. 3, pp. 181-187.
Jeschke, Marc G. ; Wolf, Steven ; DebRoy, Meelie A. ; Herndon, David. / The combination of growth hormone with hepatocyte growth factor alters the acute phase response. In: Shock. 1999 ; Vol. 12, No. 3. pp. 181-187.
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