The combination of IGF-I and KGF cDNA improves dermal and epidermal regeneration by increased VEGF expression and neovascularization

M. G. Jeschke, David Herndon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Insulin-like growth factor-I (IGF-I) and keratinocyte growth factor (KGF) cDNA gene transfer individually improves dermal and epidermal regeneration. The aim of the present study was to determine whether the combination of IGF-I plus KGF cDNA further improves wound healing and by which mechanisms these changes occur. Rats received an acute wound and were divided into four groups to receive weekly subcutaneous injections of liposomes plus Lac Z cDNA, liposomes plus IGF-I cDNA, liposomes plus KGF cDNA, or liposomes plus IGF-I/KGF cDNA. Planimetry, immunological assays, histological and immunohistochemical techniques were used to determine IGF-I, KGF, platelet-derived growth factor, fibroblast growth factor (FGF), transforming growth factor-β and vascular endothelial growth factor (VEGF) expression and different types of collagen (I, III and IV). IGF-I, KGF and their combination cDNA treatment significantly (P<0.05) accelerated re-epithelization, increased IGF-I, KGF, FGF, VEGF and collagen type IV expression, while it had no effect on collagen type I and III expression. The combination of IGF-I plus KGF cDNA increased (P<0.05) neovascularization and VEGF expression when compared to IGF-I cDNA, KGF cDNA groups and controls. In conclusion, exogenous administration of liposomal IGF-I plus KGF cDNA enhanced dermal and epidermal regeneration which is due to increased neovascularization.

Original languageEnglish (US)
Pages (from-to)1235-1242
Number of pages8
JournalGene Therapy
Volume14
Issue number16
DOIs
StatePublished - Aug 2007

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Fibroblast Growth Factor 7
Insulin-Like Growth Factor I
Vascular Endothelial Growth Factor A
Regeneration
Complementary DNA
Skin
Liposomes
Collagen Type III
Collagen Type IV
Fibroblast Growth Factors
Collagen Type I
Histological Techniques
Platelet-Derived Growth Factor
Transforming Growth Factors
Subcutaneous Injections
Wound Healing

ASJC Scopus subject areas

  • Genetics

Cite this

The combination of IGF-I and KGF cDNA improves dermal and epidermal regeneration by increased VEGF expression and neovascularization. / Jeschke, M. G.; Herndon, David.

In: Gene Therapy, Vol. 14, No. 16, 08.2007, p. 1235-1242.

Research output: Contribution to journalArticle

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