The commercial pig as a model of spontaneously-occurring osteoarthritis

Mhairi A. Macfadyen, Zoe Daniel, Sara Kelly, Tim Parr, John M. Brameld, Andrew Murton, Simon W. Jones

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Preclinical osteoarthritis models where damage occurs spontaneously may better reflect the initiation and development of human osteoarthritis. The aim was to assess the commercial pig as a model of spontaneous osteoarthritis development by examining pain-associated behaviour, joint cartilage integrity, as well as the use of porcine cartilage explants and isolated chondrocytes and osteoblasts for ex vivo and in vitro studies. Methods: Female pigs (Large white x Landrace x Duroc) were examined at different ages from 6 weeks to 3-4 years old. Lameness was assessed as a marker of pain-associated behaviour. Femorotibial joint cartilage integrity was determined by chondropathy scoring and histological staining of proteoglycan. IL-6 production and proteoglycan degradation was assessed in cartilage explants and primary porcine chondrocytes by ELISA and DMMB assay. Primary porcine osteoblasts from damaged and non-damaged joints, as determined by chondropathy scoring, were assessed for mineralisation, proliferative and mitochondrial function as a marker of metabolic capacity. Results: Pigs aged 80 weeks and older exhibited lameness. Osteoarthritic lesions in femoral condyle and tibial plateau cartilage were apparent from 40 weeks and increased in severity with age up to 3-4 years old. Cartilage from damaged joints exhibited proteoglycan loss, which positively correlated with chondropathy score. Stimulation of porcine cartilage explants and primary chondrocytes with either IL-1β or visfatin induced IL-6 production and proteoglycan degradation. Primary porcine osteoblasts from damaged joints exhibited reduced proliferative, mineralisation, and metabolic capacity. Conclusion: In conclusion, the commercial pig represents an alternative model of spontaneous osteoarthritis and an excellent source of tissue for in vitro and ex vivo studies.

Original languageEnglish (US)
Article number70
JournalBMC Musculoskeletal Disorders
Volume20
Issue number1
DOIs
StatePublished - Feb 11 2019

Fingerprint

Osteoarthritis
Swine
Cartilage
Proteoglycans
Joints
Chondrocytes
Osteoblasts
Interleukin-6
Nicotinamide Phosphoribosyltransferase
Pain
Human Development
Thigh
Interleukin-1
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Bone and Bones

Keywords

  • Chondrocyte
  • Chondropathy
  • Osteoarthritis
  • Osteoblast
  • Pig

ASJC Scopus subject areas

  • Rheumatology
  • Orthopedics and Sports Medicine

Cite this

Macfadyen, M. A., Daniel, Z., Kelly, S., Parr, T., Brameld, J. M., Murton, A., & Jones, S. W. (2019). The commercial pig as a model of spontaneously-occurring osteoarthritis. BMC Musculoskeletal Disorders, 20(1), [70]. https://doi.org/10.1186/s12891-019-2452-0

The commercial pig as a model of spontaneously-occurring osteoarthritis. / Macfadyen, Mhairi A.; Daniel, Zoe; Kelly, Sara; Parr, Tim; Brameld, John M.; Murton, Andrew; Jones, Simon W.

In: BMC Musculoskeletal Disorders, Vol. 20, No. 1, 70, 11.02.2019.

Research output: Contribution to journalArticle

Macfadyen, Mhairi A. ; Daniel, Zoe ; Kelly, Sara ; Parr, Tim ; Brameld, John M. ; Murton, Andrew ; Jones, Simon W. / The commercial pig as a model of spontaneously-occurring osteoarthritis. In: BMC Musculoskeletal Disorders. 2019 ; Vol. 20, No. 1.
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