The complete nucleotide sequence of the Popp (1967) strain of Marburg virus: a comparison with the Musoke (1980) strain

Alexander Bukreyev, V. E. Volchkov, V. M. Blinov, S. A. Dryga, S. V. Netesov

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The nucleotide sequence of genomic RNA of Marburg virus strain Popp was determined. Strain Popp was isolated in 1967 during the first filoviral outbreak. The virus was purified from blood of infected guinea pigs in which it had been maintained. The length of the determined sequence was 19112 nucleotides. Amino acid sequences of seven known virion proteins were deduced. Nucleotide and amino acid sequences were compared with those of strain Musoke of Marburg virus isolated in 1980 in Kenya and purified from Vero cells. Homology between nucleotide sequences of two strains was 93.9%. Comparisons revealed conserved and variable regions of the nucleotide and amino acid sequences. The GP, the envelope protein of the virion, was found to be the most variable protein. The greatest differences in the protein were located in the supposedly external part of the molecule. Amino acid substitutions in the L protein, the main component of viral RNA-dependent RNA polymerase, were also distributed extremely non-randomly. It was shown that the non-coding regions of the genome were more variable than the coding ones; 37.6% of nucleotide differences corresponded to the former. 72.6% of nucleotide substitutions located in the coding regions were found to be at the third codon position.

Original languageEnglish (US)
Pages (from-to)1589-1600
Number of pages12
JournalArchives of Virology
Volume140
Issue number9
DOIs
StatePublished - Sep 1995
Externally publishedYes

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Marburgvirus
Nucleotides
Amino Acid Sequence
Proteins
Virion
RNA Replicase
Vero Cells
Kenya
RNA Viruses
Viral RNA
Amino Acid Substitution
Codon
Disease Outbreaks
Guinea Pigs
Genome
Viruses

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

The complete nucleotide sequence of the Popp (1967) strain of Marburg virus : a comparison with the Musoke (1980) strain. / Bukreyev, Alexander; Volchkov, V. E.; Blinov, V. M.; Dryga, S. A.; Netesov, S. V.

In: Archives of Virology, Vol. 140, No. 9, 09.1995, p. 1589-1600.

Research output: Contribution to journalArticle

Bukreyev, Alexander ; Volchkov, V. E. ; Blinov, V. M. ; Dryga, S. A. ; Netesov, S. V. / The complete nucleotide sequence of the Popp (1967) strain of Marburg virus : a comparison with the Musoke (1980) strain. In: Archives of Virology. 1995 ; Vol. 140, No. 9. pp. 1589-1600.
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