The continuous spread of West Nile virus (WNV)

Seroprevalence in asymptomatic horses

J. Alonso-Padilla, E. Loza-Rubio, E. Escribano-Romero, L. Córdoba, S. Cuevas, F. Mejí, R. Calderón, F. Milián, A. Travassos da Rosa, Scott Weaver, J. G. Estrada-Franco, J. C. Saiz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

West Nile virus (WNV) was probably introduced in southern and northern Mexico from the USA in two independent events. Since then, WNV activity has been reported in several Mexican states bordering the USA and the Gulf of Mexico, but disease manifestations seen there in humans and equids are quite different to those observed in the USA. We have analysed WNV seroprevalence in asymptomatic, unvaccinated equids from two Mexican states where no data had been previously recorded. WNV IgG antibodies were detected in 31-6% (91/288) of equine sera from Chiapas and Puebla states (53.3% and 8.0%, respectively). Analysis by plaque reduction neutralization test (PRNT) showed good specificity (99.4%) and sensitivity (84.9%) with the ELISA results. Further analyses to detect antibodies against three different flaviviruses (WNV, St Louis encephalitis virus, Ilheus virus) by haemagglutination inhibition (HI) tests on a subset of 138 samples showed that 53% of the 83 HI-positive samples showed specific reaction to WNV. These data suggest continuous expansion of WNV through Mexico.

Original languageEnglish (US)
Pages (from-to)1163-1168
Number of pages6
JournalEpidemiology and Infection
Volume137
Issue number8
DOIs
StatePublished - 2009

Fingerprint

West Nile virus
Seroepidemiologic Studies
Horses
Mexico
Encephalitis Viruses
Gulf of Mexico
Hemagglutination Inhibition Tests
Flavivirus
Neutralization Tests
Antibodies
Hemagglutination
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Viruses
Sensitivity and Specificity
Serum

Keywords

  • Emerging viruses
  • Serology
  • Viral expansion
  • Viruses
  • Zoonoses

ASJC Scopus subject areas

  • Infectious Diseases
  • Epidemiology

Cite this

Alonso-Padilla, J., Loza-Rubio, E., Escribano-Romero, E., Córdoba, L., Cuevas, S., Mejí, F., ... Saiz, J. C. (2009). The continuous spread of West Nile virus (WNV): Seroprevalence in asymptomatic horses. Epidemiology and Infection, 137(8), 1163-1168. https://doi.org/10.1017/S0950268809002325

The continuous spread of West Nile virus (WNV) : Seroprevalence in asymptomatic horses. / Alonso-Padilla, J.; Loza-Rubio, E.; Escribano-Romero, E.; Córdoba, L.; Cuevas, S.; Mejí, F.; Calderón, R.; Milián, F.; Travassos da Rosa, A.; Weaver, Scott; Estrada-Franco, J. G.; Saiz, J. C.

In: Epidemiology and Infection, Vol. 137, No. 8, 2009, p. 1163-1168.

Research output: Contribution to journalArticle

Alonso-Padilla, J, Loza-Rubio, E, Escribano-Romero, E, Córdoba, L, Cuevas, S, Mejí, F, Calderón, R, Milián, F, Travassos da Rosa, A, Weaver, S, Estrada-Franco, JG & Saiz, JC 2009, 'The continuous spread of West Nile virus (WNV): Seroprevalence in asymptomatic horses', Epidemiology and Infection, vol. 137, no. 8, pp. 1163-1168. https://doi.org/10.1017/S0950268809002325
Alonso-Padilla J, Loza-Rubio E, Escribano-Romero E, Córdoba L, Cuevas S, Mejí F et al. The continuous spread of West Nile virus (WNV): Seroprevalence in asymptomatic horses. Epidemiology and Infection. 2009;137(8):1163-1168. https://doi.org/10.1017/S0950268809002325
Alonso-Padilla, J. ; Loza-Rubio, E. ; Escribano-Romero, E. ; Córdoba, L. ; Cuevas, S. ; Mejí, F. ; Calderón, R. ; Milián, F. ; Travassos da Rosa, A. ; Weaver, Scott ; Estrada-Franco, J. G. ; Saiz, J. C. / The continuous spread of West Nile virus (WNV) : Seroprevalence in asymptomatic horses. In: Epidemiology and Infection. 2009 ; Vol. 137, No. 8. pp. 1163-1168.
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