The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket

Hélène Malet, Bruno Coutard, Saïd Jamal, Hélène Dutartre, Nicolas Papageorgiou, Maarit Neuvonen, Tero Ahola, Naomi Forrester, Ernest A. Gould, Daniel Lafitte, Francois Ferron, Julien Lescar, Alexander E. Gorbalenya, Xavier De Lamballerie, Bruno Canard

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Macro domains (also called "X domains") constitute a protein module family present in all kingdoms of life, including viruses of the Coronaviridae and Togaviridae families. Crystal structures of the macro domain from the Chikungunya virus (an "Old World" alphavirus) and the Venezuelan equine encephalitis virus (a "New World" alphavirus) were determined at resolutions of 1.65 and 2.30 Å, respectively. These domains are active as adenosine di-phosphoribose 1″-phosphate phosphatases. Both the Chikungunya and the Venezuelan equine encephalitis virus macro domains are ADP-ribose binding modules, as revealed by structural and functional analysis. A single aspartic acid conserved through all macro domains is responsible for the specific binding of the adenine base. Sequence-unspecific binding to long, negatively charged polymers such as poly(ADP-ribose), DNA, and RNA is observed and attributed to positively charged patches outside of the active site pocket, as judged by mutagenesis and binding studies. The crystal structure of the Chikungunya virus macro domain with an RNA trimer shows a binding mode utilizing the same adenine-binding pocket as ADP-ribose, but avoiding the ADP-ribose 1″-phosphate phosphatase active site. This leaves the AMP binding site as the sole common feature in all macro domains.

Original languageEnglish (US)
Pages (from-to)6534-6545
Number of pages12
JournalJournal of Virology
Volume83
Issue number13
DOIs
StatePublished - Jul 2009

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Venezuelan Equine Encephalitis Viruses
Chikungunya virus
Venezuelan equine encephalitis virus
Adenosine Diphosphate Ribose
Alphavirus
ribose
Adenine
adenosine
crystal structure
Phosphoric Monoester Hydrolases
Adenosine
Coronaviridae
Catalytic Domain
Togaviridae
RNA
Poly Adenosine Diphosphate Ribose
Adenosine Monophosphate
adenine
Aspartic Acid
active sites

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket. / Malet, Hélène; Coutard, Bruno; Jamal, Saïd; Dutartre, Hélène; Papageorgiou, Nicolas; Neuvonen, Maarit; Ahola, Tero; Forrester, Naomi; Gould, Ernest A.; Lafitte, Daniel; Ferron, Francois; Lescar, Julien; Gorbalenya, Alexander E.; De Lamballerie, Xavier; Canard, Bruno.

In: Journal of Virology, Vol. 83, No. 13, 07.2009, p. 6534-6545.

Research output: Contribution to journalArticle

Malet, H, Coutard, B, Jamal, S, Dutartre, H, Papageorgiou, N, Neuvonen, M, Ahola, T, Forrester, N, Gould, EA, Lafitte, D, Ferron, F, Lescar, J, Gorbalenya, AE, De Lamballerie, X & Canard, B 2009, 'The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket', Journal of Virology, vol. 83, no. 13, pp. 6534-6545. https://doi.org/10.1128/JVI.00189-09
Malet, Hélène ; Coutard, Bruno ; Jamal, Saïd ; Dutartre, Hélène ; Papageorgiou, Nicolas ; Neuvonen, Maarit ; Ahola, Tero ; Forrester, Naomi ; Gould, Ernest A. ; Lafitte, Daniel ; Ferron, Francois ; Lescar, Julien ; Gorbalenya, Alexander E. ; De Lamballerie, Xavier ; Canard, Bruno. / The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket. In: Journal of Virology. 2009 ; Vol. 83, No. 13. pp. 6534-6545.
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