The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives

Yane Shih Wang, William K. Russell, Zhiyong Wang, Wei Wan, Lindsey E. Dodd, Pei Jing Pai, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

51 Scopus citations


Using evolved pyrrolysyl-tRNA synthetase-tRNAPylCUA pairs, l-phenylalanine, p-iodo-l-phenylalanine and p-bromo-l-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.

Original languageEnglish (US)
Pages (from-to)714-717
Number of pages4
JournalMolecular BioSystems
Issue number3
StatePublished - Mar 1 2011
Externally publishedYes


ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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