The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives

Yane Shih Wang, William Russell, Zhiyong Wang, Wei Wan, Lindsey E. Dodd, Pei Jing Pai, David H. Russell, Wenshe R. Liu

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Using evolved pyrrolysyl-tRNA synthetase-tRNAPylCUA pairs, l-phenylalanine, p-iodo-l-phenylalanine and p-bromo-l-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.

Original languageEnglish (US)
Pages (from-to)714-717
Number of pages4
JournalMolecular BioSystems
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

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Amino Acyl-tRNA Synthetases
Phenylalanine
Escherichia coli
Mutation
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives. / Wang, Yane Shih; Russell, William; Wang, Zhiyong; Wan, Wei; Dodd, Lindsey E.; Pai, Pei Jing; Russell, David H.; Liu, Wenshe R.

In: Molecular BioSystems, Vol. 7, No. 3, 01.03.2011, p. 714-717.

Research output: Contribution to journalArticle

Wang, Yane Shih ; Russell, William ; Wang, Zhiyong ; Wan, Wei ; Dodd, Lindsey E. ; Pai, Pei Jing ; Russell, David H. ; Liu, Wenshe R. / The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives. In: Molecular BioSystems. 2011 ; Vol. 7, No. 3. pp. 714-717.
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