The delayed induction of c-jun in apoptotic human leukemic lymphoblasts is primarily transcriptional

Feng Zhou, Rheem D. Medh, Weiping Zhang, Naseem Ansari, E. Brad Thompson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Because of their ability to induce lymphoid cell apoptosis, glucocorticoids have been used for decades to treat certain human leukemias and lymphomas. Studies presented in this paper complement our previous work demonstrating that sustained induction of the proto-oncogene c-jun plays a crucial role in the glucocorticoid-induced apoptotic pathway in CEM cells, human leukemic lymphoblasts. Results from measurements of c-jun mRNA half-life with RNase protection assays and of transcription by nuclear run-on assays indicate that, in the dexamethasone-sensitive cloned CEM-C7 cells, c-jun is induced at the transcriptional level. Consideration of time-course, however, suggested that this might be a secondary or possibly a delayed primary response. Use of cycloheximide to block protein synthesis strongly induced c-jun mRNA, suggesting that there had been relief from a labile protein repressor of transcription. Comparing the level of induction by cycloheximide with that of dexamethasone indicated that the two did not induce by an identical mechanism. The high induction by cycloheximide obscured simple interpretation of elevated c-jun mRNA levels after concomitant administration of cycloheximide and dexamethasone. This was resolved by nuclear run-on experiments, which showed that the dexamethasone induction of c-jun mRNA in this system does require protein synthesis.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume75
Issue number2-3
DOIs
StatePublished - Dec 15 2000

Fingerprint

Cycloheximide
Dexamethasone
Messenger RNA
Transcription
Glucocorticoids
Assays
jun Genes
Repressor Proteins
Ribonucleases
Half-Life
Lymphoma
Leukemia
Proteins
Lymphocytes
Apoptosis
Experiments

Keywords

  • Apoptosis
  • c-jun Gene regulation
  • CEM cells
  • Glucocorticoids
  • Lymphoid

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

The delayed induction of c-jun in apoptotic human leukemic lymphoblasts is primarily transcriptional. / Zhou, Feng; Medh, Rheem D.; Zhang, Weiping; Ansari, Naseem; Thompson, E. Brad.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 75, No. 2-3, 15.12.2000, p. 91-99.

Research output: Contribution to journalArticle

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