The developmental cycle of Ehrlichia chaffeensis in vertebrate cells

Jianzhi Zhang, Vsevolod Popov, Si Gao, David Walker, Xue Jie Yu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Ehrlichia chaffeensis, an obligatory intracellular bacterium, has two forms in mammalian cells: small dense-cored cells (DC) with dense nucleoid and larger reticulate cells (RC) with uniformly dispersed nucleoid. We have determined by electron microscopy that DC but not RC attaches to and enters into the host cells and RC but not DC multiples inside the host cells. Analysis of outer membrane protein expression by confocal microscopy showed that RC expressed the 28 kDa outer membrane protein (p28), the intermediate form, which were transforming from RC to DC, expressed both gp120 and p28, and the mature DC expressed gp120 only. The TCID50 of DC is 6 log10 higher than RC. We conclude that E.chaffeensis has a developmental cycle, in which the DC attaches to and enters into the host cells, and transforms into RC and the RC multiplies by binary fission for 48 h and then matures into DC at 72 h.

Original languageEnglish (US)
Pages (from-to)610-618
Number of pages9
JournalCellular Microbiology
Volume9
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Ehrlichia chaffeensis
Vertebrates
Membrane Proteins
Confocal microscopy
Electron microscopy
Bacteria
Cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

The developmental cycle of Ehrlichia chaffeensis in vertebrate cells. / Zhang, Jianzhi; Popov, Vsevolod; Gao, Si; Walker, David; Yu, Xue Jie.

In: Cellular Microbiology, Vol. 9, No. 3, 03.2007, p. 610-618.

Research output: Contribution to journalArticle

Zhang, Jianzhi ; Popov, Vsevolod ; Gao, Si ; Walker, David ; Yu, Xue Jie. / The developmental cycle of Ehrlichia chaffeensis in vertebrate cells. In: Cellular Microbiology. 2007 ; Vol. 9, No. 3. pp. 610-618.
@article{ffcb50cbc8744ff2abbb67b04262129f,
title = "The developmental cycle of Ehrlichia chaffeensis in vertebrate cells",
abstract = "Ehrlichia chaffeensis, an obligatory intracellular bacterium, has two forms in mammalian cells: small dense-cored cells (DC) with dense nucleoid and larger reticulate cells (RC) with uniformly dispersed nucleoid. We have determined by electron microscopy that DC but not RC attaches to and enters into the host cells and RC but not DC multiples inside the host cells. Analysis of outer membrane protein expression by confocal microscopy showed that RC expressed the 28 kDa outer membrane protein (p28), the intermediate form, which were transforming from RC to DC, expressed both gp120 and p28, and the mature DC expressed gp120 only. The TCID50 of DC is 6 log10 higher than RC. We conclude that E.chaffeensis has a developmental cycle, in which the DC attaches to and enters into the host cells, and transforms into RC and the RC multiplies by binary fission for 48 h and then matures into DC at 72 h.",
author = "Jianzhi Zhang and Vsevolod Popov and Si Gao and David Walker and Yu, {Xue Jie}",
year = "2007",
month = "3",
doi = "10.1111/j.1462-5822.2006.00812.x",
language = "English (US)",
volume = "9",
pages = "610--618",
journal = "Cellular Microbiology",
issn = "1462-5814",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - The developmental cycle of Ehrlichia chaffeensis in vertebrate cells

AU - Zhang, Jianzhi

AU - Popov, Vsevolod

AU - Gao, Si

AU - Walker, David

AU - Yu, Xue Jie

PY - 2007/3

Y1 - 2007/3

N2 - Ehrlichia chaffeensis, an obligatory intracellular bacterium, has two forms in mammalian cells: small dense-cored cells (DC) with dense nucleoid and larger reticulate cells (RC) with uniformly dispersed nucleoid. We have determined by electron microscopy that DC but not RC attaches to and enters into the host cells and RC but not DC multiples inside the host cells. Analysis of outer membrane protein expression by confocal microscopy showed that RC expressed the 28 kDa outer membrane protein (p28), the intermediate form, which were transforming from RC to DC, expressed both gp120 and p28, and the mature DC expressed gp120 only. The TCID50 of DC is 6 log10 higher than RC. We conclude that E.chaffeensis has a developmental cycle, in which the DC attaches to and enters into the host cells, and transforms into RC and the RC multiplies by binary fission for 48 h and then matures into DC at 72 h.

AB - Ehrlichia chaffeensis, an obligatory intracellular bacterium, has two forms in mammalian cells: small dense-cored cells (DC) with dense nucleoid and larger reticulate cells (RC) with uniformly dispersed nucleoid. We have determined by electron microscopy that DC but not RC attaches to and enters into the host cells and RC but not DC multiples inside the host cells. Analysis of outer membrane protein expression by confocal microscopy showed that RC expressed the 28 kDa outer membrane protein (p28), the intermediate form, which were transforming from RC to DC, expressed both gp120 and p28, and the mature DC expressed gp120 only. The TCID50 of DC is 6 log10 higher than RC. We conclude that E.chaffeensis has a developmental cycle, in which the DC attaches to and enters into the host cells, and transforms into RC and the RC multiplies by binary fission for 48 h and then matures into DC at 72 h.

UR - http://www.scopus.com/inward/record.url?scp=33846934966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846934966&partnerID=8YFLogxK

U2 - 10.1111/j.1462-5822.2006.00812.x

DO - 10.1111/j.1462-5822.2006.00812.x

M3 - Article

VL - 9

SP - 610

EP - 618

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5814

IS - 3

ER -