The Drosophila miti-mere gene, a member of the POU family, is required for the specification of the RP2/sibling lineage during neurogenesis

Krishna Moorthi Bhat, Paul Schedl

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The Drosophila POU gene miti mere (previously known as pdm2) has a complex spatial and temporal pattern of expression during early development; initially it is expressed in gap-gene-like pattern, then in 14 stripes and finally in a subset of the cells in the developing CNS and PNS. To study the function of this gene during development, we generated a 'synthetic anti-morphic mutation' by expressing a truncated version of the miti protein from a constitutive hsp83 and an inducible hsp70 promoter. We show that these Δmiti transgenes behave like classical antimorphic mutations. Using these dominant negative transgenes, together with deletions and a duplication for the gene, we show that miti is required during segmentation and neurogenesis. We have also used temperature-shift experiments with the hsp70Δmiti transgene to demonstrate that miti function in segmentation is distinct and separable from its function during neurogenesis. In segmentation, miti appears to be required in the specification of the segments A2 and A6. In the CNS, miti is required for the elaboration of the NB4-2→GMC-1→RP2/sib lineage. miti is initially required in this lineage to establish the identity of the parental ganglion mother cell, GMC-1. miti must then be down-regulated to allow the asymmetric division of GMC-1 into the RP2 and its sibling cell.

Original languageEnglish (US)
Pages (from-to)1483-1501
Number of pages19
JournalDevelopment
Volume120
Issue number6
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

Neurogenesis
Transgenes
Drosophila
Siblings
Genes
Mutation
Gene Duplication
varespladib methyl
Ganglia
Stem Cells
Temperature
Proteins

Keywords

  • Drosophila melanogaster
  • Ganglion mother cell
  • Miti-mere POU gene
  • Neuroblast stem cell
  • Neurogenesis

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

The Drosophila miti-mere gene, a member of the POU family, is required for the specification of the RP2/sibling lineage during neurogenesis. / Bhat, Krishna Moorthi; Schedl, Paul.

In: Development, Vol. 120, No. 6, 06.1994, p. 1483-1501.

Research output: Contribution to journalArticle

@article{5722069160f34c30a1dd97018a45cb5e,
title = "The Drosophila miti-mere gene, a member of the POU family, is required for the specification of the RP2/sibling lineage during neurogenesis",
abstract = "The Drosophila POU gene miti mere (previously known as pdm2) has a complex spatial and temporal pattern of expression during early development; initially it is expressed in gap-gene-like pattern, then in 14 stripes and finally in a subset of the cells in the developing CNS and PNS. To study the function of this gene during development, we generated a 'synthetic anti-morphic mutation' by expressing a truncated version of the miti protein from a constitutive hsp83 and an inducible hsp70 promoter. We show that these Δmiti transgenes behave like classical antimorphic mutations. Using these dominant negative transgenes, together with deletions and a duplication for the gene, we show that miti is required during segmentation and neurogenesis. We have also used temperature-shift experiments with the hsp70Δmiti transgene to demonstrate that miti function in segmentation is distinct and separable from its function during neurogenesis. In segmentation, miti appears to be required in the specification of the segments A2 and A6. In the CNS, miti is required for the elaboration of the NB4-2→GMC-1→RP2/sib lineage. miti is initially required in this lineage to establish the identity of the parental ganglion mother cell, GMC-1. miti must then be down-regulated to allow the asymmetric division of GMC-1 into the RP2 and its sibling cell.",
keywords = "Drosophila melanogaster, Ganglion mother cell, Miti-mere POU gene, Neuroblast stem cell, Neurogenesis",
author = "Bhat, {Krishna Moorthi} and Paul Schedl",
year = "1994",
month = "6",
language = "English (US)",
volume = "120",
pages = "1483--1501",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "6",

}

TY - JOUR

T1 - The Drosophila miti-mere gene, a member of the POU family, is required for the specification of the RP2/sibling lineage during neurogenesis

AU - Bhat, Krishna Moorthi

AU - Schedl, Paul

PY - 1994/6

Y1 - 1994/6

N2 - The Drosophila POU gene miti mere (previously known as pdm2) has a complex spatial and temporal pattern of expression during early development; initially it is expressed in gap-gene-like pattern, then in 14 stripes and finally in a subset of the cells in the developing CNS and PNS. To study the function of this gene during development, we generated a 'synthetic anti-morphic mutation' by expressing a truncated version of the miti protein from a constitutive hsp83 and an inducible hsp70 promoter. We show that these Δmiti transgenes behave like classical antimorphic mutations. Using these dominant negative transgenes, together with deletions and a duplication for the gene, we show that miti is required during segmentation and neurogenesis. We have also used temperature-shift experiments with the hsp70Δmiti transgene to demonstrate that miti function in segmentation is distinct and separable from its function during neurogenesis. In segmentation, miti appears to be required in the specification of the segments A2 and A6. In the CNS, miti is required for the elaboration of the NB4-2→GMC-1→RP2/sib lineage. miti is initially required in this lineage to establish the identity of the parental ganglion mother cell, GMC-1. miti must then be down-regulated to allow the asymmetric division of GMC-1 into the RP2 and its sibling cell.

AB - The Drosophila POU gene miti mere (previously known as pdm2) has a complex spatial and temporal pattern of expression during early development; initially it is expressed in gap-gene-like pattern, then in 14 stripes and finally in a subset of the cells in the developing CNS and PNS. To study the function of this gene during development, we generated a 'synthetic anti-morphic mutation' by expressing a truncated version of the miti protein from a constitutive hsp83 and an inducible hsp70 promoter. We show that these Δmiti transgenes behave like classical antimorphic mutations. Using these dominant negative transgenes, together with deletions and a duplication for the gene, we show that miti is required during segmentation and neurogenesis. We have also used temperature-shift experiments with the hsp70Δmiti transgene to demonstrate that miti function in segmentation is distinct and separable from its function during neurogenesis. In segmentation, miti appears to be required in the specification of the segments A2 and A6. In the CNS, miti is required for the elaboration of the NB4-2→GMC-1→RP2/sib lineage. miti is initially required in this lineage to establish the identity of the parental ganglion mother cell, GMC-1. miti must then be down-regulated to allow the asymmetric division of GMC-1 into the RP2 and its sibling cell.

KW - Drosophila melanogaster

KW - Ganglion mother cell

KW - Miti-mere POU gene

KW - Neuroblast stem cell

KW - Neurogenesis

UR - http://www.scopus.com/inward/record.url?scp=0028235599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028235599&partnerID=8YFLogxK

M3 - Article

VL - 120

SP - 1483

EP - 1501

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 6

ER -