The effect of amniotic fluid on the human omental artery in vitro

Y. P. Vedernikov, George Saade, M. Zlatnik, Dora Martin, R. E. Garfield, Gary Hankins

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

OBJECTIVE: The aim of the study was to determine the effect of amniotic fluid on the in vitro contractility of the human omental artery. STUDY DESIGN: Amniotic fluid and a segment of omentum were obtained from each of 5 patients at the time of planned cesarean delivery at normal term gestation for the indication of previous cesarean delivery. The omental artery was cleaned and cut into 3-mm rings, which were placed in 10-mL organ chambers for isometric tension recording. The chambers were filled with Krebs- Henseleit solution bubbled with 5% carbon dioxide in air and maintained at 37°C, pH 7,4. The rings were then equilibrated at 1 g passive tension for 90 minutes. The amniotic fluid was centrifuged for 10 minutes at 3000 rpm to remove all debris. Increasing volumes of supernatant (10-2000 μL) were added to the omental artery rings at baseline tone or after contraction with U46619 (10-7 mol/L) or potassium chloride (60 mmol/L) to detect contractile and relaxant effects, respectively. Time-solvent control preparations were also run in parallel. RESULTS: Amniotic fluid had no effect on the basal tone of omental artery rings. Amniotic fluid had no effect on the tension in rings previously contracted with either U46619 or potassium chloride. CONCLUSIONS: Amniotic fluid has no direct effect on isolated human omental artery. The catastrophic hemodynamic changes associated with the syndrome of amniotic fluid embolism are not due to a direct effect of circulating amniotic fluid on vascular tone but rather may be due to secondary responses.

Original languageEnglish (US)
Pages (from-to)454-456
Number of pages3
JournalAmerican Journal of Obstetrics and Gynecology
Volume180
Issue number2 I
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Amniotic Fluid
Arteries
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Potassium Chloride
Amniotic Fluid Embolism
Omentum
In Vitro Techniques
Carbon Dioxide
Blood Vessels
Hemodynamics
Air
Pregnancy

Keywords

  • Amniotic fluid
  • Amniotic fluid embolism
  • Omental artery
  • Pregnancy
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

The effect of amniotic fluid on the human omental artery in vitro. / Vedernikov, Y. P.; Saade, George; Zlatnik, M.; Martin, Dora; Garfield, R. E.; Hankins, Gary.

In: American Journal of Obstetrics and Gynecology, Vol. 180, No. 2 I, 1999, p. 454-456.

Research output: Contribution to journalArticle

Vedernikov, Y. P. ; Saade, George ; Zlatnik, M. ; Martin, Dora ; Garfield, R. E. ; Hankins, Gary. / The effect of amniotic fluid on the human omental artery in vitro. In: American Journal of Obstetrics and Gynecology. 1999 ; Vol. 180, No. 2 I. pp. 454-456.
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