The effect of antimicrobial agents on the induction of tumour necrosis factor by alveolar macrophages in vitro in response to endotoxin

Fiemu E. Nwariaku, Kendra L. McIntyre, Patricia J. Sikes, William Mileski

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Alveolar macrophages from New Zealand white rabbits were incubated with twice the MIC of amikacin, ciprofloxacin, aztreonam, ceftazidime and imipenem and exposed to either 104, 105 or 106 cfu/ml live Pseudomonas aeruginosa ATCC 27853 or 0.1, 1 or 10 mg/L purified lipopolysaccharide (LPS) derived from P. aeruginosa to determine the effects of different classes of antimicrobial agent on production of tumour necrosis factor (TNF). Incubation of macrophages with ciprofloxacin and amikacin resulted in less TNF activity after exposure to live P. aeruginosa than was found for saline, aztreonam, ceftazidime or imipenem (P < 0.05). However, no significant differences were found between any of the agents after macrophages had been exposed to purified LPS. Different antimicrobial agents therefore appear to exert different effects in vitro on the TNF response of macrophages to bacterial stimulation.

Original languageEnglish (US)
Pages (from-to)265-267
Number of pages3
JournalJournal of Antimicrobial Chemotherapy
Volume39
Issue number2
DOIs
StatePublished - 1997
Externally publishedYes

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Alveolar Macrophages
Anti-Infective Agents
Endotoxins
Pseudomonas aeruginosa
Aztreonam
Ceftazidime
Amikacin
Tumor Necrosis Factor-alpha
Imipenem
Macrophages
Ciprofloxacin
Lipopolysaccharides
Rabbits
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

The effect of antimicrobial agents on the induction of tumour necrosis factor by alveolar macrophages in vitro in response to endotoxin. / Nwariaku, Fiemu E.; McIntyre, Kendra L.; Sikes, Patricia J.; Mileski, William.

In: Journal of Antimicrobial Chemotherapy, Vol. 39, No. 2, 1997, p. 265-267.

Research output: Contribution to journalArticle

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