The effect of blood transfusion on susceptibility to bacterial infection in genetically defined mouse models

Tonyia Eaves-Pyles, J. Wesley Alexander

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Blood transfusions suppress immune function and increase susceptibility to infection, but the effects are not consistent. Study Design and Methods: Genetically defined mouse strains with the same or different haplotypes were used as blood transfusion recipients and donors. Transfused animals were subjected to cecal ligation and puncture (CLP) and followed for survival or were injected intravenously with Candida albicans to follow clearance of the Candida from the kidneys. Results: BALB/c (H-2(d)) mice transfused with C3H/HeJ (H-2(k)) or DBA/2 (H-2(d)) blood followed by CLP showed significantly lower survival (7 and 10%) than mice transfused with syngeneic blood (61%) or saline controls (56%). Lower survival was also observed in C3H/HeJ (H-2(k)) mice transfused with BALB/c (H 2(d)) blood and subjected to CLP (25%) compared with syngeneic transfusion (80%) or saline controls (70%). C57BL/6J (H-2(b)) mice showed minimal increases in mortality after CLP after transfusion with blood from C3H/HeJ (H-2(k)) (60% survival), DBA/2 (H-2(d)) (70% survival), or BALB/c (H-2(d)) mice (90% survival). When C. albicans was infused intravenously into transfused mice, a similar pattern of altered resistance to infection was found. Conclusion: The ability of blood transfusions to increase susceptibility to bacterial infection appears to be dependent on genetic factors unrelated to the major haplotype.

Original languageEnglish (US)
Pages (from-to)894-898
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume43
Issue number6
DOIs
StatePublished - Dec 1997
Externally publishedYes

Fingerprint

Bacterial Infections
Blood Transfusion
Punctures
Ligation
Candida albicans
Haplotypes
Infection
Blood Donors
Candida
Kidney
Mortality

Keywords

  • Blood transfusion
  • Genetic resistance
  • Immune function
  • Infection

ASJC Scopus subject areas

  • Surgery

Cite this

@article{d18c5b2aca09461f8e88c71fdcb07ad5,
title = "The effect of blood transfusion on susceptibility to bacterial infection in genetically defined mouse models",
abstract = "Background: Blood transfusions suppress immune function and increase susceptibility to infection, but the effects are not consistent. Study Design and Methods: Genetically defined mouse strains with the same or different haplotypes were used as blood transfusion recipients and donors. Transfused animals were subjected to cecal ligation and puncture (CLP) and followed for survival or were injected intravenously with Candida albicans to follow clearance of the Candida from the kidneys. Results: BALB/c (H-2(d)) mice transfused with C3H/HeJ (H-2(k)) or DBA/2 (H-2(d)) blood followed by CLP showed significantly lower survival (7 and 10{\%}) than mice transfused with syngeneic blood (61{\%}) or saline controls (56{\%}). Lower survival was also observed in C3H/HeJ (H-2(k)) mice transfused with BALB/c (H 2(d)) blood and subjected to CLP (25{\%}) compared with syngeneic transfusion (80{\%}) or saline controls (70{\%}). C57BL/6J (H-2(b)) mice showed minimal increases in mortality after CLP after transfusion with blood from C3H/HeJ (H-2(k)) (60{\%} survival), DBA/2 (H-2(d)) (70{\%} survival), or BALB/c (H-2(d)) mice (90{\%} survival). When C. albicans was infused intravenously into transfused mice, a similar pattern of altered resistance to infection was found. Conclusion: The ability of blood transfusions to increase susceptibility to bacterial infection appears to be dependent on genetic factors unrelated to the major haplotype.",
keywords = "Blood transfusion, Genetic resistance, Immune function, Infection",
author = "Tonyia Eaves-Pyles and Alexander, {J. Wesley}",
year = "1997",
month = "12",
doi = "10.1097/00005373-199712000-00004",
language = "English (US)",
volume = "43",
pages = "894--898",
journal = "Journal of Trauma and Acute Care Surgery",
issn = "2163-0755",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - The effect of blood transfusion on susceptibility to bacterial infection in genetically defined mouse models

AU - Eaves-Pyles, Tonyia

AU - Alexander, J. Wesley

PY - 1997/12

Y1 - 1997/12

N2 - Background: Blood transfusions suppress immune function and increase susceptibility to infection, but the effects are not consistent. Study Design and Methods: Genetically defined mouse strains with the same or different haplotypes were used as blood transfusion recipients and donors. Transfused animals were subjected to cecal ligation and puncture (CLP) and followed for survival or were injected intravenously with Candida albicans to follow clearance of the Candida from the kidneys. Results: BALB/c (H-2(d)) mice transfused with C3H/HeJ (H-2(k)) or DBA/2 (H-2(d)) blood followed by CLP showed significantly lower survival (7 and 10%) than mice transfused with syngeneic blood (61%) or saline controls (56%). Lower survival was also observed in C3H/HeJ (H-2(k)) mice transfused with BALB/c (H 2(d)) blood and subjected to CLP (25%) compared with syngeneic transfusion (80%) or saline controls (70%). C57BL/6J (H-2(b)) mice showed minimal increases in mortality after CLP after transfusion with blood from C3H/HeJ (H-2(k)) (60% survival), DBA/2 (H-2(d)) (70% survival), or BALB/c (H-2(d)) mice (90% survival). When C. albicans was infused intravenously into transfused mice, a similar pattern of altered resistance to infection was found. Conclusion: The ability of blood transfusions to increase susceptibility to bacterial infection appears to be dependent on genetic factors unrelated to the major haplotype.

AB - Background: Blood transfusions suppress immune function and increase susceptibility to infection, but the effects are not consistent. Study Design and Methods: Genetically defined mouse strains with the same or different haplotypes were used as blood transfusion recipients and donors. Transfused animals were subjected to cecal ligation and puncture (CLP) and followed for survival or were injected intravenously with Candida albicans to follow clearance of the Candida from the kidneys. Results: BALB/c (H-2(d)) mice transfused with C3H/HeJ (H-2(k)) or DBA/2 (H-2(d)) blood followed by CLP showed significantly lower survival (7 and 10%) than mice transfused with syngeneic blood (61%) or saline controls (56%). Lower survival was also observed in C3H/HeJ (H-2(k)) mice transfused with BALB/c (H 2(d)) blood and subjected to CLP (25%) compared with syngeneic transfusion (80%) or saline controls (70%). C57BL/6J (H-2(b)) mice showed minimal increases in mortality after CLP after transfusion with blood from C3H/HeJ (H-2(k)) (60% survival), DBA/2 (H-2(d)) (70% survival), or BALB/c (H-2(d)) mice (90% survival). When C. albicans was infused intravenously into transfused mice, a similar pattern of altered resistance to infection was found. Conclusion: The ability of blood transfusions to increase susceptibility to bacterial infection appears to be dependent on genetic factors unrelated to the major haplotype.

KW - Blood transfusion

KW - Genetic resistance

KW - Immune function

KW - Infection

UR - http://www.scopus.com/inward/record.url?scp=0031459235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031459235&partnerID=8YFLogxK

U2 - 10.1097/00005373-199712000-00004

DO - 10.1097/00005373-199712000-00004

M3 - Article

VL - 43

SP - 894

EP - 898

JO - Journal of Trauma and Acute Care Surgery

JF - Journal of Trauma and Acute Care Surgery

SN - 2163-0755

IS - 6

ER -