The effect of class II major histocompatibility complex expression on adherence of Helicobacter pylori and induction of apoptosis in gastric epithelial cells: A mechanism for T helper cell type 1-mediated damage

Xuejun Fan, Sheila E. Crowe, Simon Behar, Harshani Gunasena, Gang Ye, Helene Haeberle, Nancy Van Houten, William K. Gourley, Peter B. Ernst, Victor Reyes

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Helicobacter pylori infection is associated with gastric epithelial damage, including apoptosis, ulceration, and cancer. Although bacterial factors and the host response are believed to contribute to gastric disease, no receptor has been identified that explains how the bacteria attach and signal the host cell to undergo apoptosis. Using H. pylori as 'bait' to capture receptor proteins in solubilized membranes of gastric epithelial cells, class II major histocompatibility complex (MHC) molecules were identified as a possible receptor. Signaling through class II MHC molecules leading to the induction of apoptosis was confirmed using cross-linking IgM antibodies to surface class II MHC molecules. Moreover, binding of H. pylori and the induction of apoptosis were inhibited by antibodies recognizing class II MHC. Since type 1 T helper cells are present during infection and produce interferon (IFN)-γ, which increases class II MHC expression, gastric epithelial cell lines were exposed to H. pylori in the presence or absence of IFN-γ. IFN-γ, increased the attachment of the bacteria as well as the induction of apoptosis in gastric epithelial cells. In contrast to MHC II- negative cell lines, H. pylori induced apoptosis in cells expressing class II MHC molecules constitutively or after gene transfection. These data describe a novel receptor for H. pylori and provide a mechanism by which bacteria and the host response interact in the pathogenesis of gastric epithelial cell damage.

Original languageEnglish (US)
Pages (from-to)1659-1669
Number of pages11
JournalJournal of Experimental Medicine
Volume187
Issue number10
DOIs
StatePublished - May 18 1998

Fingerprint

Th1 Cells
Major Histocompatibility Complex
Helicobacter pylori
Stomach
Epithelial Cells
Apoptosis
Interferons
Bacteria
Stomach Diseases
Cell Line
Immunoglobulin Isotypes
Helicobacter Infections
Transfection
Immunoglobulin M
Membranes
Antibodies
Infection
Genes

Keywords

  • Apoptosis
  • Epithelium
  • Gastric
  • Helicobacter pylori
  • T helper cell type 1

ASJC Scopus subject areas

  • Immunology

Cite this

The effect of class II major histocompatibility complex expression on adherence of Helicobacter pylori and induction of apoptosis in gastric epithelial cells : A mechanism for T helper cell type 1-mediated damage. / Fan, Xuejun; Crowe, Sheila E.; Behar, Simon; Gunasena, Harshani; Ye, Gang; Haeberle, Helene; Van Houten, Nancy; Gourley, William K.; Ernst, Peter B.; Reyes, Victor.

In: Journal of Experimental Medicine, Vol. 187, No. 10, 18.05.1998, p. 1659-1669.

Research output: Contribution to journalArticle

Fan, Xuejun ; Crowe, Sheila E. ; Behar, Simon ; Gunasena, Harshani ; Ye, Gang ; Haeberle, Helene ; Van Houten, Nancy ; Gourley, William K. ; Ernst, Peter B. ; Reyes, Victor. / The effect of class II major histocompatibility complex expression on adherence of Helicobacter pylori and induction of apoptosis in gastric epithelial cells : A mechanism for T helper cell type 1-mediated damage. In: Journal of Experimental Medicine. 1998 ; Vol. 187, No. 10. pp. 1659-1669.
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