The effect of copper on human and bovine lens and on human cultured lens epithelium enzymes

Yogesh C. Awasthi, Steven P. Miller, D. V. Arya, Satish Srivastava

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effect of physiological levels of copper on the glycolytic and shunt pathway enzymes of glucose metabolism in human and bovine lens and on cultured lens epithelium has been studied. In human and bovine lens homogenates, hexokinase, phosphofructokinase, pyruvate kinase, glyceraldehydephosphate dehydrogenase, phosphoglycerate kinase and 6-phosphogluconate dehydrogenase were almost completely inhibited in the presence of 50 μm-copper. Aldolase, glucose-6-phosphate dehydrogenase and triose phosphate isomerase were inhibited 78, 50 and 36% respectively. The inhibition of most of the enzymes was not significantly reversed by the addition of EDTA. The inhibition of enzyme activity was also not reversed by dialysis of the copper-treated enzyme against phosphate buffer. Similar results were obtained when human cultured lens epithelium was used as the enzyme source. The studies indicate that if the increased amount of copper present in senile cataractous lenses is in the free form, it can significantly impair glucose metabolism in the lens.

Original languageEnglish (US)
Pages (from-to)251-257
Number of pages7
JournalExperimental Eye Research
Volume21
Issue number3
DOIs
StatePublished - 1975

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Lenses
Copper
Epithelium
Enzymes
Triose-Phosphate Isomerase
Phosphogluconate Dehydrogenase
Phosphoglycerate Kinase
Glucose
Phosphofructokinases
Fructose-Bisphosphate Aldolase
Glyceraldehyde-3-Phosphate Dehydrogenases
Hexokinase
Pyruvate Kinase
Glucosephosphate Dehydrogenase
Edetic Acid
Dialysis
Buffers
Phosphates

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

The effect of copper on human and bovine lens and on human cultured lens epithelium enzymes. / Awasthi, Yogesh C.; Miller, Steven P.; Arya, D. V.; Srivastava, Satish.

In: Experimental Eye Research, Vol. 21, No. 3, 1975, p. 251-257.

Research output: Contribution to journalArticle

Awasthi, Yogesh C. ; Miller, Steven P. ; Arya, D. V. ; Srivastava, Satish. / The effect of copper on human and bovine lens and on human cultured lens epithelium enzymes. In: Experimental Eye Research. 1975 ; Vol. 21, No. 3. pp. 251-257.
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