The effect of deletion of a conserved 11 nucleotide sequence on mouse hepatitis virus RNA replication

J. F. Repass, Shinji Makino

Research output: Contribution to journalArticle

Abstract

A conserved 11 nt sequence present at near the 3' end of mouse hepatitis virus (MHV) genomic RNA binds to host proteins and is important for MHV RNA replication (Yu and Leibowitz, 1995). To better understand the role of this 11 nt sequence in positive-strand MHV RNA replication, we examined whether positive-strand MHV DI RNAs from negative-strand DI RNA transcripts lacking the 11 nt sequence were synthesized in MHV-infected cells. Positive-strand DI RNAs did not accumulate efficiently, indicating that the conserved 11 nt sequence was necessary for positive-strand MHV RNA synthesis.

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume440
StatePublished - 1998
Externally publishedYes

Fingerprint

Murine hepatitis virus
Virus Replication
Viruses
Nucleotides
RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{e2bf27083f834dd094ea4ca4bda9fd19,
title = "The effect of deletion of a conserved 11 nucleotide sequence on mouse hepatitis virus RNA replication",
abstract = "A conserved 11 nt sequence present at near the 3' end of mouse hepatitis virus (MHV) genomic RNA binds to host proteins and is important for MHV RNA replication (Yu and Leibowitz, 1995). To better understand the role of this 11 nt sequence in positive-strand MHV RNA replication, we examined whether positive-strand MHV DI RNAs from negative-strand DI RNA transcripts lacking the 11 nt sequence were synthesized in MHV-infected cells. Positive-strand DI RNAs did not accumulate efficiently, indicating that the conserved 11 nt sequence was necessary for positive-strand MHV RNA synthesis.",
author = "Repass, {J. F.} and Shinji Makino",
year = "1998",
language = "English (US)",
volume = "440",
pages = "247--252",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - The effect of deletion of a conserved 11 nucleotide sequence on mouse hepatitis virus RNA replication

AU - Repass, J. F.

AU - Makino, Shinji

PY - 1998

Y1 - 1998

N2 - A conserved 11 nt sequence present at near the 3' end of mouse hepatitis virus (MHV) genomic RNA binds to host proteins and is important for MHV RNA replication (Yu and Leibowitz, 1995). To better understand the role of this 11 nt sequence in positive-strand MHV RNA replication, we examined whether positive-strand MHV DI RNAs from negative-strand DI RNA transcripts lacking the 11 nt sequence were synthesized in MHV-infected cells. Positive-strand DI RNAs did not accumulate efficiently, indicating that the conserved 11 nt sequence was necessary for positive-strand MHV RNA synthesis.

AB - A conserved 11 nt sequence present at near the 3' end of mouse hepatitis virus (MHV) genomic RNA binds to host proteins and is important for MHV RNA replication (Yu and Leibowitz, 1995). To better understand the role of this 11 nt sequence in positive-strand MHV RNA replication, we examined whether positive-strand MHV DI RNAs from negative-strand DI RNA transcripts lacking the 11 nt sequence were synthesized in MHV-infected cells. Positive-strand DI RNAs did not accumulate efficiently, indicating that the conserved 11 nt sequence was necessary for positive-strand MHV RNA synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0031720832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031720832&partnerID=8YFLogxK

M3 - Article

VL - 440

SP - 247

EP - 252

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -