The effect of exogenous recombinant rat-interleukin-10 on oxygen toxicity in neonatal rats

D. V. Dallas, S. E. Keeney, H. E. Rudloff, D. Tarrant, K. H. Palkowetz, F. C. Schmalstieg

Research output: Contribution to journalArticle

Abstract

Hyperoxic lung injury has been implicated in the pathogenesis of bronchopulmonary dysplasia. While the mechanisms for this injury remain unclear, reactive oxygen species and inflammatory cytokines are thought to be important mediators in the inflammation leading to this injury. In preliminary studies we have found that neonatal rats, like human neonates, have a profound developmental delay in the production of IL-10 in the blood and lung. The role of IL-10 in the protection of oxidative injury was therefore investigated. Recombinant rat IL-10 (rrIL-10) was prepared using a baculovirus expression vector in SF9 insect cells (concentration 1.0 μg/ml). Sprague Dawley neonatal rats < 24 h old were placed in > 98% O2 or air. After 4 days of O2 or air exposure, neonates in both groups were treated daily with rrIL-10 (250 U/kg) intraperitoneally (i.p.), rrIL-10 (250 U/kg) intranasally (i.n.) or saline intranasally (i.n.). Survival for 14 days was compared: Survival (O2) 7 days 10 days 14 days Saline i.n. 1/3 (33%) 1/3 (33%) 0 % rrIL-10 i.n. 1/4 (25%) 1/4 (25%) 0% rrIL-10 i.p. 2/3 (66%) 2/3 (66%) 2/3 (66%) After 14 days of > 98% O2 there was 66% survival of rrIL-10 i.p. rats compared to 0% survival in the saline or rrIL-10 i.n. O2 groups. There was 100% survival in all air groups. We further investigated rrIL-10 i.p. (500 U/kg) vs saline controls i.p. starting with daily injections after 4 days of > 98% O2 or air. After 10 days of O2, survival of rr IL-10 i.p. animals was 6/6 (100%), significantly higher compared to 1/6 (17%) survival of saline i.p. controls in O2. We conclude that rrIL-10 given intraperitoneally may significantly enhance survival of neonatal rats during hyperoxia.

Original languageEnglish (US)
JournalJournal of Investigative Medicine
Volume47
Issue number2
StatePublished - 1999

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Interleukin-10
Toxicity
Rats
Oxygen
Survival
Air
Wounds and Injuries
Inflammation Mediators
Bronchopulmonary Dysplasia
Hyperoxia
Baculoviridae
Lung Injury
Sprague Dawley Rats
Insects
Reactive Oxygen Species
Animals
Blood
Cytokines
Lung
Injections

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dallas, D. V., Keeney, S. E., Rudloff, H. E., Tarrant, D., Palkowetz, K. H., & Schmalstieg, F. C. (1999). The effect of exogenous recombinant rat-interleukin-10 on oxygen toxicity in neonatal rats. Journal of Investigative Medicine, 47(2).

The effect of exogenous recombinant rat-interleukin-10 on oxygen toxicity in neonatal rats. / Dallas, D. V.; Keeney, S. E.; Rudloff, H. E.; Tarrant, D.; Palkowetz, K. H.; Schmalstieg, F. C.

In: Journal of Investigative Medicine, Vol. 47, No. 2, 1999.

Research output: Contribution to journalArticle

Dallas, DV, Keeney, SE, Rudloff, HE, Tarrant, D, Palkowetz, KH & Schmalstieg, FC 1999, 'The effect of exogenous recombinant rat-interleukin-10 on oxygen toxicity in neonatal rats', Journal of Investigative Medicine, vol. 47, no. 2.
Dallas DV, Keeney SE, Rudloff HE, Tarrant D, Palkowetz KH, Schmalstieg FC. The effect of exogenous recombinant rat-interleukin-10 on oxygen toxicity in neonatal rats. Journal of Investigative Medicine. 1999;47(2).
Dallas, D. V. ; Keeney, S. E. ; Rudloff, H. E. ; Tarrant, D. ; Palkowetz, K. H. ; Schmalstieg, F. C. / The effect of exogenous recombinant rat-interleukin-10 on oxygen toxicity in neonatal rats. In: Journal of Investigative Medicine. 1999 ; Vol. 47, No. 2.
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abstract = "Hyperoxic lung injury has been implicated in the pathogenesis of bronchopulmonary dysplasia. While the mechanisms for this injury remain unclear, reactive oxygen species and inflammatory cytokines are thought to be important mediators in the inflammation leading to this injury. In preliminary studies we have found that neonatal rats, like human neonates, have a profound developmental delay in the production of IL-10 in the blood and lung. The role of IL-10 in the protection of oxidative injury was therefore investigated. Recombinant rat IL-10 (rrIL-10) was prepared using a baculovirus expression vector in SF9 insect cells (concentration 1.0 μg/ml). Sprague Dawley neonatal rats < 24 h old were placed in > 98{\%} O2 or air. After 4 days of O2 or air exposure, neonates in both groups were treated daily with rrIL-10 (250 U/kg) intraperitoneally (i.p.), rrIL-10 (250 U/kg) intranasally (i.n.) or saline intranasally (i.n.). Survival for 14 days was compared: Survival (O2) 7 days 10 days 14 days Saline i.n. 1/3 (33{\%}) 1/3 (33{\%}) 0 {\%} rrIL-10 i.n. 1/4 (25{\%}) 1/4 (25{\%}) 0{\%} rrIL-10 i.p. 2/3 (66{\%}) 2/3 (66{\%}) 2/3 (66{\%}) After 14 days of > 98{\%} O2 there was 66{\%} survival of rrIL-10 i.p. rats compared to 0{\%} survival in the saline or rrIL-10 i.n. O2 groups. There was 100{\%} survival in all air groups. We further investigated rrIL-10 i.p. (500 U/kg) vs saline controls i.p. starting with daily injections after 4 days of > 98{\%} O2 or air. After 10 days of O2, survival of rr IL-10 i.p. animals was 6/6 (100{\%}), significantly higher compared to 1/6 (17{\%}) survival of saline i.p. controls in O2. We conclude that rrIL-10 given intraperitoneally may significantly enhance survival of neonatal rats during hyperoxia.",
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AU - Dallas, D. V.

AU - Keeney, S. E.

AU - Rudloff, H. E.

AU - Tarrant, D.

AU - Palkowetz, K. H.

AU - Schmalstieg, F. C.

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N2 - Hyperoxic lung injury has been implicated in the pathogenesis of bronchopulmonary dysplasia. While the mechanisms for this injury remain unclear, reactive oxygen species and inflammatory cytokines are thought to be important mediators in the inflammation leading to this injury. In preliminary studies we have found that neonatal rats, like human neonates, have a profound developmental delay in the production of IL-10 in the blood and lung. The role of IL-10 in the protection of oxidative injury was therefore investigated. Recombinant rat IL-10 (rrIL-10) was prepared using a baculovirus expression vector in SF9 insect cells (concentration 1.0 μg/ml). Sprague Dawley neonatal rats < 24 h old were placed in > 98% O2 or air. After 4 days of O2 or air exposure, neonates in both groups were treated daily with rrIL-10 (250 U/kg) intraperitoneally (i.p.), rrIL-10 (250 U/kg) intranasally (i.n.) or saline intranasally (i.n.). Survival for 14 days was compared: Survival (O2) 7 days 10 days 14 days Saline i.n. 1/3 (33%) 1/3 (33%) 0 % rrIL-10 i.n. 1/4 (25%) 1/4 (25%) 0% rrIL-10 i.p. 2/3 (66%) 2/3 (66%) 2/3 (66%) After 14 days of > 98% O2 there was 66% survival of rrIL-10 i.p. rats compared to 0% survival in the saline or rrIL-10 i.n. O2 groups. There was 100% survival in all air groups. We further investigated rrIL-10 i.p. (500 U/kg) vs saline controls i.p. starting with daily injections after 4 days of > 98% O2 or air. After 10 days of O2, survival of rr IL-10 i.p. animals was 6/6 (100%), significantly higher compared to 1/6 (17%) survival of saline i.p. controls in O2. We conclude that rrIL-10 given intraperitoneally may significantly enhance survival of neonatal rats during hyperoxia.

AB - Hyperoxic lung injury has been implicated in the pathogenesis of bronchopulmonary dysplasia. While the mechanisms for this injury remain unclear, reactive oxygen species and inflammatory cytokines are thought to be important mediators in the inflammation leading to this injury. In preliminary studies we have found that neonatal rats, like human neonates, have a profound developmental delay in the production of IL-10 in the blood and lung. The role of IL-10 in the protection of oxidative injury was therefore investigated. Recombinant rat IL-10 (rrIL-10) was prepared using a baculovirus expression vector in SF9 insect cells (concentration 1.0 μg/ml). Sprague Dawley neonatal rats < 24 h old were placed in > 98% O2 or air. After 4 days of O2 or air exposure, neonates in both groups were treated daily with rrIL-10 (250 U/kg) intraperitoneally (i.p.), rrIL-10 (250 U/kg) intranasally (i.n.) or saline intranasally (i.n.). Survival for 14 days was compared: Survival (O2) 7 days 10 days 14 days Saline i.n. 1/3 (33%) 1/3 (33%) 0 % rrIL-10 i.n. 1/4 (25%) 1/4 (25%) 0% rrIL-10 i.p. 2/3 (66%) 2/3 (66%) 2/3 (66%) After 14 days of > 98% O2 there was 66% survival of rrIL-10 i.p. rats compared to 0% survival in the saline or rrIL-10 i.n. O2 groups. There was 100% survival in all air groups. We further investigated rrIL-10 i.p. (500 U/kg) vs saline controls i.p. starting with daily injections after 4 days of > 98% O2 or air. After 10 days of O2, survival of rr IL-10 i.p. animals was 6/6 (100%), significantly higher compared to 1/6 (17%) survival of saline i.p. controls in O2. We conclude that rrIL-10 given intraperitoneally may significantly enhance survival of neonatal rats during hyperoxia.

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