The effect of gastrin on growth of human stomach cancer cells

Jin Ishizuka, Jean Martinez, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Gastrin is known as a trophic factor for some stomach and colorectal cancer cells; however, the roles of gastrin receptors and the intracellular signal transduction pathways by which gastrin regulates cell growth are still unknown. The authors examined the effect of synthetic human gastrin-17 on growth of human stomach cancer cells (the parent line, AGS-P, and two different clones, AGS-10 and ACS-12), which were established (and have been maintained) in our laboratory. Gastrin stimulated growth of AGS-P and AGS-10 cells, which have gastrin receptors, in a dose-dependent fashion. A highly selective gastrin receptor antagonist, JMV 320, inhibited the growth-stimulatory effect of gastrin on AGS-P cells in a dose-dependent fashion. Concentrations of gastrin (10-8 to 10-6 M), which stimulated growth of AGS-P cells, did not affect either cyclic adenosine monophosphate production or phosphatidylinositol hydrolysis. Gastrin (10-11 to 10-5 M) mobilized calcium from the intracellular organelles to increase intracellular calcium level in AGS-P cells. The AGS-12 clone has no gastrin receptors, and gastrin did not affect growth or mobilization of intracellular calcium in these cells. Our findings indicate that gastrin stimulates growth of AGS cells through a mechanism that involves binding to specific gastrin receptors that are linked to the system for mobilization of intracellular calcium.

Original languageEnglish (US)
Pages (from-to)528-534
Number of pages7
JournalAnnals of Surgery
Volume215
Issue number5
StatePublished - 1992

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Gastrins
Cholecystokinin B Receptor
Stomach Neoplasms
Growth
Calcium
Clone Cells
Gastrin-Secreting Cells
Phosphatidylinositols
Organelles
Cyclic AMP
Colorectal Neoplasms
Signal Transduction
Hydrolysis
Cell Line

ASJC Scopus subject areas

  • Surgery

Cite this

Ishizuka, J., Martinez, J., Townsend, C., & Thompson, J. C. (1992). The effect of gastrin on growth of human stomach cancer cells. Annals of Surgery, 215(5), 528-534.

The effect of gastrin on growth of human stomach cancer cells. / Ishizuka, Jin; Martinez, Jean; Townsend, Courtney; Thompson, James C.

In: Annals of Surgery, Vol. 215, No. 5, 1992, p. 528-534.

Research output: Contribution to journalArticle

Ishizuka, J, Martinez, J, Townsend, C & Thompson, JC 1992, 'The effect of gastrin on growth of human stomach cancer cells', Annals of Surgery, vol. 215, no. 5, pp. 528-534.
Ishizuka J, Martinez J, Townsend C, Thompson JC. The effect of gastrin on growth of human stomach cancer cells. Annals of Surgery. 1992;215(5):528-534.
Ishizuka, Jin ; Martinez, Jean ; Townsend, Courtney ; Thompson, James C. / The effect of gastrin on growth of human stomach cancer cells. In: Annals of Surgery. 1992 ; Vol. 215, No. 5. pp. 528-534.
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