The effect of leukocyte depletion on smoke inhalation injury in sheep

J. O. Basadre, K. Sugi, D. L. Traber, L. D. Traber, G. D. Niehaus, David Herndon

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Leukocytes and the production of oxygen radicals and proteolytic enzymes have been implicated in the pathogenesis of lung injury after smoke inhalation. We investigated the mechanism responsible for this form of pulmonary damage in chronically prepared sheep previously made leukopenic with intra-arterial infusions of nitrogen mustard (mechlorethamine hydrochloride). A control air insufflated group (sham: n = 6), a cotton smoke insufflated group (smoke: n = 12), and a leukopenic cotton smoke insufflation group (smoked + depleted: n = 6) were compared. Although both smoke insufflation groups had equivalent smoke exposure, which was indexed by carboxyhemoglobin, the smoked + depleted group had significant attenuation in the increases in pulmonary artery pressure, pulmonary vascular resistance, and pulmonary lymph flow. The PaO2 to FiO2 ratio (P:F) did not fall to the same extent, nor was there a fall in PaO2. The production of oxygen radicals, which was measured as plasma-conjugated dienes, and the consumption of antiprotease, as measured by α2-macroglobulin levels in lung lymph, were not changed in the smoked + depleted group, whereas it was elevated in the smoked group. We conclude that circulating leukocytes and the release of oxygen radicals and proteolytic enzymes contribute to the lung injury, pulmonary microvascular permeability increase, and pulmonary edema seen after smoke inhalation.

Original languageEnglish (US)
Pages (from-to)208-215
Number of pages8
JournalSurgery
Volume104
Issue number2
StatePublished - 1988

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Smoke Inhalation Injury
Smoke
Sheep
Leukocytes
Mechlorethamine
Reactive Oxygen Species
Lung
Insufflation
Lung Injury
Lymph
Inhalation
Peptide Hydrolases
Carboxyhemoglobin
Intra Arterial Infusions
Macroglobulins
Capillary Permeability
Pulmonary Edema
Protease Inhibitors
Vascular Resistance
Pulmonary Artery

ASJC Scopus subject areas

  • Surgery

Cite this

Basadre, J. O., Sugi, K., Traber, D. L., Traber, L. D., Niehaus, G. D., & Herndon, D. (1988). The effect of leukocyte depletion on smoke inhalation injury in sheep. Surgery, 104(2), 208-215.

The effect of leukocyte depletion on smoke inhalation injury in sheep. / Basadre, J. O.; Sugi, K.; Traber, D. L.; Traber, L. D.; Niehaus, G. D.; Herndon, David.

In: Surgery, Vol. 104, No. 2, 1988, p. 208-215.

Research output: Contribution to journalArticle

Basadre, JO, Sugi, K, Traber, DL, Traber, LD, Niehaus, GD & Herndon, D 1988, 'The effect of leukocyte depletion on smoke inhalation injury in sheep', Surgery, vol. 104, no. 2, pp. 208-215.
Basadre JO, Sugi K, Traber DL, Traber LD, Niehaus GD, Herndon D. The effect of leukocyte depletion on smoke inhalation injury in sheep. Surgery. 1988;104(2):208-215.
Basadre, J. O. ; Sugi, K. ; Traber, D. L. ; Traber, L. D. ; Niehaus, G. D. ; Herndon, David. / The effect of leukocyte depletion on smoke inhalation injury in sheep. In: Surgery. 1988 ; Vol. 104, No. 2. pp. 208-215.
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