The effect of methadone and buprenorphine on human placental aromatase

Olga L. Zharikova, Sujal V. Deshmukh, Tatiana N. Nanovskaya, Gary D.V. Hankins, Mahmoud S. Ahmed

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Methadone and buprenorphine (BUP) are used for treatment of the pregnant opiate addict. CYP19/aromatase is the major placental enzyme responsible for the metabolism of methadone to 2-ethylidine-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and BUP to norbuprenorphine (norBUP). The aim of this investigation was to determine the effects of methadone and BUP on the activity of placental microsomal aromatase in the conversion of its endogenous substrates testosterone to 17β-estradiol (E2) and 16α-hydroxytestosterone (16-OHT) to estriol (E3). The conversion of testosterone and 16-OHT by human placental microsomes exhibited saturation kinetics, and the apparent Km values were 0.2 ± 1 and 6 ± 3 μM, respectively. Vmax values for E2 and E3 formation were 70 ± 16 and 28 ± 10 pmol/mg protein min, respectively. Also, data obtained revealed that methadone and BUP are competitive inhibitors of testosterone conversion to E2 and 16-OHT to E3. The K i for methadone inhibition of E2 and E3 formation were 393 ± 144 and 53 ± 28 μM, respectively, and for BUP the Ki was 36 ± 9 and 6 ± 1 μM. The higher potency of the two opiates and their metabolites in inhibiting E3 formation is in agreement with the lower affinity of 16-OHT than testosterone to aromatase. Moreover, the metabolites EDDP and norBUP were weaker inhibitors of aromatase than their parent compounds. The determined inhibition constants of methadone and BUP for E3 formation by a cDNA-expressed CYP19 preparation were similar to those for placental microsomes. Therefore, data reported here suggest that methadone, BUP, and their metabolites are inhibitors of androgen aromatization in the placental biosynthesis of estrogens.

Original languageEnglish (US)
Pages (from-to)1255-1264
Number of pages10
JournalBiochemical Pharmacology
Volume71
Issue number8
DOIs
StatePublished - Apr 14 2006

Keywords

  • Buprenorphine (BUP)
  • CYP19/aromatase
  • Estrogen formation
  • Human placenta
  • Methadone
  • Pregnant opiate addict

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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