The effect of prepregnancy obesity and sFlt-1-induced preeclampsia-like syndrome on fetal programming of adult vascular function in a mouse model

Benjamin D. Byers, Ancizar Betancourt, Fangxian Lu, Gary Hankins, Monica Longo, George Saade, Egle Bytautiene

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: The purpose of this study was to test the hypothesis that prepregnancy obesity and soluble fms-like tyrosine kinase-1 (sFlt-1)-induced preeclampsia lead to altered vascular function in the offspring later in life. Study Design: CD-1 female mice were placed on a low-fat (LF) or high-fat (HF) diet before mating. On day 8 of pregnancy, the HF mice were injected with adenovirus that carried either sFlt-1 (HF sFlt-1) or murine immunoglobulin G2α Fc fragment (HF mFc). LF dams received saline solution. After being weaned, all offspring were placed on a standard diet. At 3 months of age, the carotid artery was isolated for in vitro vascular reactivity studies. Results: Among male offspring, the response to phenylephrine was significantly lower in the HF sFlt-1 group. The response to serotonin in males and to thromboxane in females was lower in the HF sFlt-1 and HF mFc groups. In females, the HF sFlt-1 and LF groups displayed less relaxation to acetylcholine. The response to phenylephrine was significantly lower in females than males in the HF mFc and LF groups. The response to thromboxane was significantly lower in the HF sFlt-1 females, compared with males. Conclusion: Prepregnancy obesity and preeclampsia alter fetal programming of adult vascular function. The mechanism is complex and gender specific.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume200
Issue number4
DOIs
StatePublished - Apr 2009

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Vascular Endothelial Growth Factor Receptor-1
Fetal Development
Pre-Eclampsia
Blood Vessels
Obesity
Fats
Thromboxanes
Phenylephrine
Immunoglobulin Fc Fragments
High Fat Diet
Carotid Arteries
Adenoviridae
Sodium Chloride
Acetylcholine

Keywords

  • CD-1
  • fetal programming
  • mice
  • obesity
  • preeclampsia
  • sFlt-1

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

The effect of prepregnancy obesity and sFlt-1-induced preeclampsia-like syndrome on fetal programming of adult vascular function in a mouse model. / Byers, Benjamin D.; Betancourt, Ancizar; Lu, Fangxian; Hankins, Gary; Longo, Monica; Saade, George; Bytautiene, Egle.

In: American Journal of Obstetrics and Gynecology, Vol. 200, No. 4, 04.2009.

Research output: Contribution to journalArticle

Byers, Benjamin D. ; Betancourt, Ancizar ; Lu, Fangxian ; Hankins, Gary ; Longo, Monica ; Saade, George ; Bytautiene, Egle. / The effect of prepregnancy obesity and sFlt-1-induced preeclampsia-like syndrome on fetal programming of adult vascular function in a mouse model. In: American Journal of Obstetrics and Gynecology. 2009 ; Vol. 200, No. 4.
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