The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON

Scott R. Silva, Yekaterina Y. Zaytseva, Lindsey N. Jackson, Eun Y. Lee, Heidi L. Weiss, Kanika Bowen-Jallow, Courtney Townsend, B. Mark Evers

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Carcinoid tumors are associated with the carcinoid syndrome, a set of symptoms resulting from the peptide and amine products, including serotonin, secreted from the cancer cells. The purpose of this study was to investigate the relationship between the phosphatidylinositol-3-kinaselprotein kinase B (PI3K/Akt) inhibitor PTEN (phosphatase and tensin homolog deleted on chromosome ten) and serotonin synthesis and secretion in the carcinoid cancer cell line BON. Materials and Methods: PTEN was inhibited by pharmacological and molecular approaches, and the resultant secretion of serotonin and expression of tryptophan hydroxylase 1 (TPH1), the rate-limiting enzyme in serotonin synthesis, was assessed. Results: Inhibition of PTEN in vitro, with concomitant increased Akt signaling, resulted in decreased secretion of serotonin, as well as decreased serotonin synthesis, as confirmed by reduced expression of TPH1. Inhibition of PTEN in BON cells in an animal model resulted in decreased serum serotonin. Conclusion: By inhibiting signaling through Akt, PTEN indirectly promotes serotonin synthesis and secretion.

Original languageEnglish (US)
Pages (from-to)1153-1160
Number of pages8
JournalAnticancer Research
Volume31
Issue number4
StatePublished - Apr 2011

Fingerprint

Carcinoid Tumor
Serotonin
Cell Line
Tryptophan Hydroxylase
PTEN Phosphohydrolase
Phosphatidylinositol 3-Kinase
Phosphatidylinositol 3-Kinases
Amines
Neoplasms
Animal Models
Chromosomes
Pharmacology
Peptides
Enzymes
Serum

Keywords

  • Carcinoid
  • PTEN
  • Serotonin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Silva, S. R., Zaytseva, Y. Y., Jackson, L. N., Lee, E. Y., Weiss, H. L., Bowen-Jallow, K., ... Evers, B. M. (2011). The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON. Anticancer Research, 31(4), 1153-1160.

The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON. / Silva, Scott R.; Zaytseva, Yekaterina Y.; Jackson, Lindsey N.; Lee, Eun Y.; Weiss, Heidi L.; Bowen-Jallow, Kanika; Townsend, Courtney; Evers, B. Mark.

In: Anticancer Research, Vol. 31, No. 4, 04.2011, p. 1153-1160.

Research output: Contribution to journalArticle

Silva, SR, Zaytseva, YY, Jackson, LN, Lee, EY, Weiss, HL, Bowen-Jallow, K, Townsend, C & Evers, BM 2011, 'The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON', Anticancer Research, vol. 31, no. 4, pp. 1153-1160.
Silva SR, Zaytseva YY, Jackson LN, Lee EY, Weiss HL, Bowen-Jallow K et al. The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON. Anticancer Research. 2011 Apr;31(4):1153-1160.
Silva, Scott R. ; Zaytseva, Yekaterina Y. ; Jackson, Lindsey N. ; Lee, Eun Y. ; Weiss, Heidi L. ; Bowen-Jallow, Kanika ; Townsend, Courtney ; Evers, B. Mark. / The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON. In: Anticancer Research. 2011 ; Vol. 31, No. 4. pp. 1153-1160.
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