The effect of track structure on cell inactivation and chromosome damage at a constant let of 120 keV/μm

E. H. Goodwin, S. M. Bailey, D. J. Chen, Michael Cornforth

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The influence of track structure on chromosome damage and cell inactivation are being investigated. Plateau-phase normal human fibroblast cultures were irradiated with gamma rays, and He, Ne and Ar ions. Particle velocities were chosen so that all beams had an LET of 120 keV/μm. In this constant-LET experimental design, the radial distribution of excitations and ionizations about the particle track is the most significant variable. Using premature chromosome condensation, chromatin breaks were measured at two time points, promptly after irradiation and after a prolonged incubation to allow for repair. These measurements give an indication of both initial chromosomal damage and also residual damage that is either not repaired or is misrepaired. Survival was measured under the same conditions. Results indicate that the RBEs for both cell inactivation and, to a lesser extent, chromosome damage decrease as particle energy increases.

Original languageEnglish (US)
Pages (from-to)93-98
Number of pages6
JournalAdvances in Space Research
Volume18
Issue number1-2
StatePublished - 1996

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chromosomes
Chromosomes
deactivation
chromosome
damage
cells
Fibroblasts
Cell culture
Gamma rays
particle tracks
Design of experiments
chromatin
Ionization
Condensation
fibroblasts
Repair
particle energy
radial distribution
Irradiation
experimental design

ASJC Scopus subject areas

  • Space and Planetary Science
  • Astronomy and Astrophysics

Cite this

The effect of track structure on cell inactivation and chromosome damage at a constant let of 120 keV/μm. / Goodwin, E. H.; Bailey, S. M.; Chen, D. J.; Cornforth, Michael.

In: Advances in Space Research, Vol. 18, No. 1-2, 1996, p. 93-98.

Research output: Contribution to journalArticle

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