The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats

Ghaffar Shokouhi, R. Shane Tubbs, Mohammadali Mohajel Shoja, Leila Roshangar, Mehran Mesgari, Amir Ghorbanihaghjo, Naser Ahmadi, Farzam Sheikhzadeh, Jafar S. Rad

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The potential role of exercise in preventing the age-related spontaneous peripheral neuropathy has not been studied. We examined the effects of long-term aerobic exercise training on lipid peroxidation, Schwann cell (SC) apoptosis and ultrastructural changes in the sciatic nerve of rats during aging. Three groups of 12-week old Wistar rats ran on a treadmill for 6, 9 and 12 months (exercise trained (ET) group, n = 10 each) according to an exercise training program targeted at a speed of 22 m/min (at 7° incline), 60 min/day, 6 days/week. Three corresponding groups of untrained rats were used as the controls (sedentary (SED) group). At the end of each period, sciatic nerve biopsies were performed, and processed for biochemical, immunohistochemical and ultrastructural analyses. The results showed that aging was associated with an increased level of nerve malondialdehyde (MDA, marker of lipid peroxidation) and a higher number of SC apoptosis in SED group. The SED group showed irregular nerve fibers with thin myelin sheaths and areas of myelin-axon detachment. However, the ET group had significantly diminished nerve lipid peroxidation and SC apoptosis. In the ET group, nerve fibers had a thick myelin sheath with frequent folding. These findings suggest that aerobic exercise training protects peripheral nerves by attenuating oxidative reactions, and preserving SCs and myelin sheath from pathologic changes, which occur during normal aging.

Original languageEnglish (US)
Pages (from-to)840-846
Number of pages7
JournalLife Sciences
Volume82
Issue number15-16
DOIs
StatePublished - Apr 9 2008
Externally publishedYes

Fingerprint

Schwann Cells
Sciatic Nerve
Lipid Peroxidation
Rats
Myelin Sheath
Aging of materials
Cells
Exercise
Apoptosis
Lipids
Exercise equipment
Nerve Fibers
Fibers
Biopsy
Malondialdehyde
Peripheral Nervous System Diseases
Peripheral Nerves
Axons
Wistar Rats
Education

Keywords

  • Aging
  • Apoptosis
  • Exercise
  • Lipid peroxidation
  • Neuropathy
  • Sciatic nerve
  • Treadmill

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Shokouhi, G., Tubbs, R. S., Mohajel Shoja, M., Roshangar, L., Mesgari, M., Ghorbanihaghjo, A., ... Rad, J. S. (2008). The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats. Life Sciences, 82(15-16), 840-846. https://doi.org/10.1016/j.lfs.2008.01.018

The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats. / Shokouhi, Ghaffar; Tubbs, R. Shane; Mohajel Shoja, Mohammadali; Roshangar, Leila; Mesgari, Mehran; Ghorbanihaghjo, Amir; Ahmadi, Naser; Sheikhzadeh, Farzam; Rad, Jafar S.

In: Life Sciences, Vol. 82, No. 15-16, 09.04.2008, p. 840-846.

Research output: Contribution to journalArticle

Shokouhi, G, Tubbs, RS, Mohajel Shoja, M, Roshangar, L, Mesgari, M, Ghorbanihaghjo, A, Ahmadi, N, Sheikhzadeh, F & Rad, JS 2008, 'The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats', Life Sciences, vol. 82, no. 15-16, pp. 840-846. https://doi.org/10.1016/j.lfs.2008.01.018
Shokouhi, Ghaffar ; Tubbs, R. Shane ; Mohajel Shoja, Mohammadali ; Roshangar, Leila ; Mesgari, Mehran ; Ghorbanihaghjo, Amir ; Ahmadi, Naser ; Sheikhzadeh, Farzam ; Rad, Jafar S. / The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats. In: Life Sciences. 2008 ; Vol. 82, No. 15-16. pp. 840-846.
@article{56bf042b223546009415aaf3872c66b8,
title = "The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats",
abstract = "The potential role of exercise in preventing the age-related spontaneous peripheral neuropathy has not been studied. We examined the effects of long-term aerobic exercise training on lipid peroxidation, Schwann cell (SC) apoptosis and ultrastructural changes in the sciatic nerve of rats during aging. Three groups of 12-week old Wistar rats ran on a treadmill for 6, 9 and 12 months (exercise trained (ET) group, n = 10 each) according to an exercise training program targeted at a speed of 22 m/min (at 7° incline), 60 min/day, 6 days/week. Three corresponding groups of untrained rats were used as the controls (sedentary (SED) group). At the end of each period, sciatic nerve biopsies were performed, and processed for biochemical, immunohistochemical and ultrastructural analyses. The results showed that aging was associated with an increased level of nerve malondialdehyde (MDA, marker of lipid peroxidation) and a higher number of SC apoptosis in SED group. The SED group showed irregular nerve fibers with thin myelin sheaths and areas of myelin-axon detachment. However, the ET group had significantly diminished nerve lipid peroxidation and SC apoptosis. In the ET group, nerve fibers had a thick myelin sheath with frequent folding. These findings suggest that aerobic exercise training protects peripheral nerves by attenuating oxidative reactions, and preserving SCs and myelin sheath from pathologic changes, which occur during normal aging.",
keywords = "Aging, Apoptosis, Exercise, Lipid peroxidation, Neuropathy, Sciatic nerve, Treadmill",
author = "Ghaffar Shokouhi and Tubbs, {R. Shane} and {Mohajel Shoja}, Mohammadali and Leila Roshangar and Mehran Mesgari and Amir Ghorbanihaghjo and Naser Ahmadi and Farzam Sheikhzadeh and Rad, {Jafar S.}",
year = "2008",
month = "4",
day = "9",
doi = "10.1016/j.lfs.2008.01.018",
language = "English (US)",
volume = "82",
pages = "840--846",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "15-16",

}

TY - JOUR

T1 - The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats

AU - Shokouhi, Ghaffar

AU - Tubbs, R. Shane

AU - Mohajel Shoja, Mohammadali

AU - Roshangar, Leila

AU - Mesgari, Mehran

AU - Ghorbanihaghjo, Amir

AU - Ahmadi, Naser

AU - Sheikhzadeh, Farzam

AU - Rad, Jafar S.

PY - 2008/4/9

Y1 - 2008/4/9

N2 - The potential role of exercise in preventing the age-related spontaneous peripheral neuropathy has not been studied. We examined the effects of long-term aerobic exercise training on lipid peroxidation, Schwann cell (SC) apoptosis and ultrastructural changes in the sciatic nerve of rats during aging. Three groups of 12-week old Wistar rats ran on a treadmill for 6, 9 and 12 months (exercise trained (ET) group, n = 10 each) according to an exercise training program targeted at a speed of 22 m/min (at 7° incline), 60 min/day, 6 days/week. Three corresponding groups of untrained rats were used as the controls (sedentary (SED) group). At the end of each period, sciatic nerve biopsies were performed, and processed for biochemical, immunohistochemical and ultrastructural analyses. The results showed that aging was associated with an increased level of nerve malondialdehyde (MDA, marker of lipid peroxidation) and a higher number of SC apoptosis in SED group. The SED group showed irregular nerve fibers with thin myelin sheaths and areas of myelin-axon detachment. However, the ET group had significantly diminished nerve lipid peroxidation and SC apoptosis. In the ET group, nerve fibers had a thick myelin sheath with frequent folding. These findings suggest that aerobic exercise training protects peripheral nerves by attenuating oxidative reactions, and preserving SCs and myelin sheath from pathologic changes, which occur during normal aging.

AB - The potential role of exercise in preventing the age-related spontaneous peripheral neuropathy has not been studied. We examined the effects of long-term aerobic exercise training on lipid peroxidation, Schwann cell (SC) apoptosis and ultrastructural changes in the sciatic nerve of rats during aging. Three groups of 12-week old Wistar rats ran on a treadmill for 6, 9 and 12 months (exercise trained (ET) group, n = 10 each) according to an exercise training program targeted at a speed of 22 m/min (at 7° incline), 60 min/day, 6 days/week. Three corresponding groups of untrained rats were used as the controls (sedentary (SED) group). At the end of each period, sciatic nerve biopsies were performed, and processed for biochemical, immunohistochemical and ultrastructural analyses. The results showed that aging was associated with an increased level of nerve malondialdehyde (MDA, marker of lipid peroxidation) and a higher number of SC apoptosis in SED group. The SED group showed irregular nerve fibers with thin myelin sheaths and areas of myelin-axon detachment. However, the ET group had significantly diminished nerve lipid peroxidation and SC apoptosis. In the ET group, nerve fibers had a thick myelin sheath with frequent folding. These findings suggest that aerobic exercise training protects peripheral nerves by attenuating oxidative reactions, and preserving SCs and myelin sheath from pathologic changes, which occur during normal aging.

KW - Aging

KW - Apoptosis

KW - Exercise

KW - Lipid peroxidation

KW - Neuropathy

KW - Sciatic nerve

KW - Treadmill

UR - http://www.scopus.com/inward/record.url?scp=41149137540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41149137540&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2008.01.018

DO - 10.1016/j.lfs.2008.01.018

M3 - Article

VL - 82

SP - 840

EP - 846

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 15-16

ER -