The effects of an electromagnetic field on the boundary tissue of the seminiferous tubules of the rat: A light and transmission electron microscope study

A. A. Khaki, R. S. Tubbs, Mohammadali Mohajel Shoja, J. S. Rad, A. Khaki, R. M. Farahani, S. Zarrintan, T. C. Nag

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Human beings are unavoidably exposed to ambient electromagnetic fields (EMF) generated from various electrical devices and from power transmission lines. Controversy exists about the effects of EMF on various organs. One of the critical issues is that EMF may adversely affect the reproductive system. In order to examine this 30 rat pups were exposed to 50 Hz EMF (non-ionising radiation) during in utero development (approximately 3 weeks) and postnatal life (5 weeks). Groups of exposed rats were subsequently left in an environment free of EMF in order to observe recovery, if any, from the changes induced by EMF on the boundary tissue of the seminiferous tubules. The materials were processed and observed under a light and a transmission electron microscope. In the experimental rats boundary tissue was found disrupted at various layers. This tissue showed infoldings, which were perhaps due to the loss of collagen and reticular fibrils from the inner and outer non-cellular layers. The outer non-cellular layer, which was thinner than that of the control, was stripped away from the myoid cell layer in multiple regions, giving a "blister-like" appearance. The myoid cells showed fewer polyribosomes, pinocytotic vesicles and glycogen granules. Most mitochondria were found to lack cristae. The connections between individual myoid cells were apparently lost. There were signs of recovery in the boundary tissue following withdrawal from EMF exposure. These results suggest that EMF exposure may cause profound changes in the boundary tissue of the seminiferous tubules. Therefore exposure to EMF may result in pathological changes that lead to subfertility and infertility.

Original languageEnglish (US)
Pages (from-to)188-194
Number of pages7
JournalFolia Morphologica
Volume65
Issue number3
StatePublished - Aug 1 2006
Externally publishedYes

Keywords

  • Boundary tissue
  • Electromagnetic fields
  • Electron microscopy
  • Rats
  • Seminiferous tubules
  • Testes

ASJC Scopus subject areas

  • Anatomy
  • Histology

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