The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats

T. Okawa, A. S. Syal, Y. P. Vedernikov, George Saade, K. Chwalisz, R. E. Garfield

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

OBJECTIVE: The object was to compare the effects of nitric oxide on isolated uterus and aorta of pregnant rats. STUDY DESIGN: Rings of uterus and thoracic aorta without endothelium from Sprague-Dawley rats at mid and late gestation were used for isometric tension recording. The concentration- response curve for diethylamine/nitric oxide was studied in the presence or absence of oxyhemoglobin (10-5 mol/L), or oxyhemoglobin was added after the response to diethylamine/nitric oxide. RESULTS: Diethylamine/nitric oxide concentration dependently inhibited uterine contractions, and the effect was attenuated by previous treatment with oxyhemoglobin at mid gestation (n = 8). The effects were negligible at late gestation (n = 8). The relaxation of aortic rings by diethylamine/nitric oxide and its attenuation by previous treatment with oxyhemoglobin were similar at mid (n = 6) and late (n = 6) gestation. The sensitivity of aortic rings to diethylamine/nitric oxide is significantly higher than that of uterine rings. Oxyhemoglobin partly restored inhibited diethylamine/nitric oxide phenylephrine tension in aortic rings and had no effect on diethylamine/nitric oxide-inhibited uterine rings. CONCLUSIONS: Uterine smooth muscle is less sensitive to nitric oxide than is aortic smooth muscle. Nitric oxide sensitivity of rat uterus but not aorta decreases toward term.

Original languageEnglish (US)
Pages (from-to)721-726
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume179
Issue number3 I
DOIs
StatePublished - 1998

Fingerprint

Nitric Oxide
Oxyhemoglobins
Uterus
Pregnancy
Smooth Muscle
Aorta
Uterine Contraction
Myometrium
Phenylephrine
diethylamine
Thoracic Aorta
Endothelium
Sprague Dawley Rats
Arterial Pressure

Keywords

  • Aorta
  • Myometrium
  • Nitric oxide
  • Oxyhemoglobin
  • Pregnancy

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats. / Okawa, T.; Syal, A. S.; Vedernikov, Y. P.; Saade, George; Chwalisz, K.; Garfield, R. E.

In: American Journal of Obstetrics and Gynecology, Vol. 179, No. 3 I, 1998, p. 721-726.

Research output: Contribution to journalArticle

Okawa, T. ; Syal, A. S. ; Vedernikov, Y. P. ; Saade, George ; Chwalisz, K. ; Garfield, R. E. / The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats. In: American Journal of Obstetrics and Gynecology. 1998 ; Vol. 179, No. 3 I. pp. 721-726.
@article{b2ad149cbef14a648def4485a2de5796,
title = "The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats",
abstract = "OBJECTIVE: The object was to compare the effects of nitric oxide on isolated uterus and aorta of pregnant rats. STUDY DESIGN: Rings of uterus and thoracic aorta without endothelium from Sprague-Dawley rats at mid and late gestation were used for isometric tension recording. The concentration- response curve for diethylamine/nitric oxide was studied in the presence or absence of oxyhemoglobin (10-5 mol/L), or oxyhemoglobin was added after the response to diethylamine/nitric oxide. RESULTS: Diethylamine/nitric oxide concentration dependently inhibited uterine contractions, and the effect was attenuated by previous treatment with oxyhemoglobin at mid gestation (n = 8). The effects were negligible at late gestation (n = 8). The relaxation of aortic rings by diethylamine/nitric oxide and its attenuation by previous treatment with oxyhemoglobin were similar at mid (n = 6) and late (n = 6) gestation. The sensitivity of aortic rings to diethylamine/nitric oxide is significantly higher than that of uterine rings. Oxyhemoglobin partly restored inhibited diethylamine/nitric oxide phenylephrine tension in aortic rings and had no effect on diethylamine/nitric oxide-inhibited uterine rings. CONCLUSIONS: Uterine smooth muscle is less sensitive to nitric oxide than is aortic smooth muscle. Nitric oxide sensitivity of rat uterus but not aorta decreases toward term.",
keywords = "Aorta, Myometrium, Nitric oxide, Oxyhemoglobin, Pregnancy",
author = "T. Okawa and Syal, {A. S.} and Vedernikov, {Y. P.} and George Saade and K. Chwalisz and Garfield, {R. E.}",
year = "1998",
doi = "10.1016/S0002-9378(98)70071-7",
language = "English (US)",
volume = "179",
pages = "721--726",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "3 I",

}

TY - JOUR

T1 - The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats

AU - Okawa, T.

AU - Syal, A. S.

AU - Vedernikov, Y. P.

AU - Saade, George

AU - Chwalisz, K.

AU - Garfield, R. E.

PY - 1998

Y1 - 1998

N2 - OBJECTIVE: The object was to compare the effects of nitric oxide on isolated uterus and aorta of pregnant rats. STUDY DESIGN: Rings of uterus and thoracic aorta without endothelium from Sprague-Dawley rats at mid and late gestation were used for isometric tension recording. The concentration- response curve for diethylamine/nitric oxide was studied in the presence or absence of oxyhemoglobin (10-5 mol/L), or oxyhemoglobin was added after the response to diethylamine/nitric oxide. RESULTS: Diethylamine/nitric oxide concentration dependently inhibited uterine contractions, and the effect was attenuated by previous treatment with oxyhemoglobin at mid gestation (n = 8). The effects were negligible at late gestation (n = 8). The relaxation of aortic rings by diethylamine/nitric oxide and its attenuation by previous treatment with oxyhemoglobin were similar at mid (n = 6) and late (n = 6) gestation. The sensitivity of aortic rings to diethylamine/nitric oxide is significantly higher than that of uterine rings. Oxyhemoglobin partly restored inhibited diethylamine/nitric oxide phenylephrine tension in aortic rings and had no effect on diethylamine/nitric oxide-inhibited uterine rings. CONCLUSIONS: Uterine smooth muscle is less sensitive to nitric oxide than is aortic smooth muscle. Nitric oxide sensitivity of rat uterus but not aorta decreases toward term.

AB - OBJECTIVE: The object was to compare the effects of nitric oxide on isolated uterus and aorta of pregnant rats. STUDY DESIGN: Rings of uterus and thoracic aorta without endothelium from Sprague-Dawley rats at mid and late gestation were used for isometric tension recording. The concentration- response curve for diethylamine/nitric oxide was studied in the presence or absence of oxyhemoglobin (10-5 mol/L), or oxyhemoglobin was added after the response to diethylamine/nitric oxide. RESULTS: Diethylamine/nitric oxide concentration dependently inhibited uterine contractions, and the effect was attenuated by previous treatment with oxyhemoglobin at mid gestation (n = 8). The effects were negligible at late gestation (n = 8). The relaxation of aortic rings by diethylamine/nitric oxide and its attenuation by previous treatment with oxyhemoglobin were similar at mid (n = 6) and late (n = 6) gestation. The sensitivity of aortic rings to diethylamine/nitric oxide is significantly higher than that of uterine rings. Oxyhemoglobin partly restored inhibited diethylamine/nitric oxide phenylephrine tension in aortic rings and had no effect on diethylamine/nitric oxide-inhibited uterine rings. CONCLUSIONS: Uterine smooth muscle is less sensitive to nitric oxide than is aortic smooth muscle. Nitric oxide sensitivity of rat uterus but not aorta decreases toward term.

KW - Aorta

KW - Myometrium

KW - Nitric oxide

KW - Oxyhemoglobin

KW - Pregnancy

UR - http://www.scopus.com/inward/record.url?scp=0031693979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031693979&partnerID=8YFLogxK

U2 - 10.1016/S0002-9378(98)70071-7

DO - 10.1016/S0002-9378(98)70071-7

M3 - Article

VL - 179

SP - 721

EP - 726

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 3 I

ER -