The effects of sepsis and endotoxemia on gut glutamine metabolism

W. W. Souba, K. Herskowitz, Vicki Klimberg, R. M. Salloum, D. A. Plumley, T. C. Flynn, E. M. Copeland

Research output: Contribution to journalArticle

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Abstract

The effects of sepsis on gut glutamine (GLN) metabolism were studied to gain further insights into the regulation of the altered glutamine metabolism that characterizes critical illnesses. Studies were done in laboratory rats and in hospitalized patients. The human studies were done in seven healthy surgical patients (controls) and six septic patients who underwent laparotomy. Radial artery and portal vein samples were obtained during operation and were analyzed for GLN and oxygen content. Despite no reduction in arterial glutamine concentration in the septic patients, gut glutamine extraction was diminished by 75% (12.0% ± 1.6% in controls vs. 2.8% ± 0.8% in septic patients, p < 0.01). Similarly gut oxygen extracton was diminished by nearly 50% in the septic patients (p < 0.05). To further investigate these abnormalities, endotoxin (10 mg/kg intraperitoneally) or saline (controls) was administered to adult rats 12 hours before cannulation of the carotid artery and portal vein. The arterial GLN concentration was increased by 13% in the endotoxin-treated animals (p < 0.05) but gut glutamine uptake was diminished by 46% (526 ± 82 nmol/100 g BW/minute in controls vs. 282 ± 45 in endotoxin, p < 0.01). Simultaneously gut glutaminase activity was diminished by 30% (p < 0.01) and intestinal glutamate release fell by two thirds. Blood cultures were negative in control animals (0 of 20), but were positive in 25% of endotoxemic animals (6 of 24) for gram-negative rods (p = 0.019). Sepsis and endotoxemia impair gut glutamine metabolism. This impairment may be etiologic in the breakdown of the gut mucosal barrier and in the development of bacterial translocation.

Original languageEnglish (US)
Pages (from-to)543-551
Number of pages9
JournalAnnals of Surgery
Volume211
Issue number5
StatePublished - 1990
Externally publishedYes

Fingerprint

Endotoxemia
Glutamine
Sepsis
Endotoxins
Portal Vein
Oxygen
Glutaminase
Bacterial Translocation
Radial Artery
Carotid Arteries
Critical Illness
Catheterization
Laparotomy
Glutamic Acid

ASJC Scopus subject areas

  • Surgery

Cite this

Souba, W. W., Herskowitz, K., Klimberg, V., Salloum, R. M., Plumley, D. A., Flynn, T. C., & Copeland, E. M. (1990). The effects of sepsis and endotoxemia on gut glutamine metabolism. Annals of Surgery, 211(5), 543-551.

The effects of sepsis and endotoxemia on gut glutamine metabolism. / Souba, W. W.; Herskowitz, K.; Klimberg, Vicki; Salloum, R. M.; Plumley, D. A.; Flynn, T. C.; Copeland, E. M.

In: Annals of Surgery, Vol. 211, No. 5, 1990, p. 543-551.

Research output: Contribution to journalArticle

Souba, WW, Herskowitz, K, Klimberg, V, Salloum, RM, Plumley, DA, Flynn, TC & Copeland, EM 1990, 'The effects of sepsis and endotoxemia on gut glutamine metabolism', Annals of Surgery, vol. 211, no. 5, pp. 543-551.
Souba WW, Herskowitz K, Klimberg V, Salloum RM, Plumley DA, Flynn TC et al. The effects of sepsis and endotoxemia on gut glutamine metabolism. Annals of Surgery. 1990;211(5):543-551.
Souba, W. W. ; Herskowitz, K. ; Klimberg, Vicki ; Salloum, R. M. ; Plumley, D. A. ; Flynn, T. C. ; Copeland, E. M. / The effects of sepsis and endotoxemia on gut glutamine metabolism. In: Annals of Surgery. 1990 ; Vol. 211, No. 5. pp. 543-551.
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abstract = "The effects of sepsis on gut glutamine (GLN) metabolism were studied to gain further insights into the regulation of the altered glutamine metabolism that characterizes critical illnesses. Studies were done in laboratory rats and in hospitalized patients. The human studies were done in seven healthy surgical patients (controls) and six septic patients who underwent laparotomy. Radial artery and portal vein samples were obtained during operation and were analyzed for GLN and oxygen content. Despite no reduction in arterial glutamine concentration in the septic patients, gut glutamine extraction was diminished by 75{\%} (12.0{\%} ± 1.6{\%} in controls vs. 2.8{\%} ± 0.8{\%} in septic patients, p < 0.01). Similarly gut oxygen extracton was diminished by nearly 50{\%} in the septic patients (p < 0.05). To further investigate these abnormalities, endotoxin (10 mg/kg intraperitoneally) or saline (controls) was administered to adult rats 12 hours before cannulation of the carotid artery and portal vein. The arterial GLN concentration was increased by 13{\%} in the endotoxin-treated animals (p < 0.05) but gut glutamine uptake was diminished by 46{\%} (526 ± 82 nmol/100 g BW/minute in controls vs. 282 ± 45 in endotoxin, p < 0.01). Simultaneously gut glutaminase activity was diminished by 30{\%} (p < 0.01) and intestinal glutamate release fell by two thirds. Blood cultures were negative in control animals (0 of 20), but were positive in 25{\%} of endotoxemic animals (6 of 24) for gram-negative rods (p = 0.019). Sepsis and endotoxemia impair gut glutamine metabolism. This impairment may be etiologic in the breakdown of the gut mucosal barrier and in the development of bacterial translocation.",
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AU - Plumley, D. A.

AU - Flynn, T. C.

AU - Copeland, E. M.

PY - 1990

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N2 - The effects of sepsis on gut glutamine (GLN) metabolism were studied to gain further insights into the regulation of the altered glutamine metabolism that characterizes critical illnesses. Studies were done in laboratory rats and in hospitalized patients. The human studies were done in seven healthy surgical patients (controls) and six septic patients who underwent laparotomy. Radial artery and portal vein samples were obtained during operation and were analyzed for GLN and oxygen content. Despite no reduction in arterial glutamine concentration in the septic patients, gut glutamine extraction was diminished by 75% (12.0% ± 1.6% in controls vs. 2.8% ± 0.8% in septic patients, p < 0.01). Similarly gut oxygen extracton was diminished by nearly 50% in the septic patients (p < 0.05). To further investigate these abnormalities, endotoxin (10 mg/kg intraperitoneally) or saline (controls) was administered to adult rats 12 hours before cannulation of the carotid artery and portal vein. The arterial GLN concentration was increased by 13% in the endotoxin-treated animals (p < 0.05) but gut glutamine uptake was diminished by 46% (526 ± 82 nmol/100 g BW/minute in controls vs. 282 ± 45 in endotoxin, p < 0.01). Simultaneously gut glutaminase activity was diminished by 30% (p < 0.01) and intestinal glutamate release fell by two thirds. Blood cultures were negative in control animals (0 of 20), but were positive in 25% of endotoxemic animals (6 of 24) for gram-negative rods (p = 0.019). Sepsis and endotoxemia impair gut glutamine metabolism. This impairment may be etiologic in the breakdown of the gut mucosal barrier and in the development of bacterial translocation.

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