The endogenous lectin RL-29 is transynaptically induced in dorsal horn neurons following peripheral neuropathy in the rat

Adrian A. Cameron, Patrick M. Dougherty, Christopher J. Garrison, William D. Willis, Susan M. Carlton

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Neurons containing immunoreactivity to the endogenous lactose-binding lectin, RL-29, were examined in the L4 segment of the spinal cord of rats with an experimental neuropathy. The cells appeared by 5 days and were also present at 14 and 28 days postoperatively. All neurons were found in the dorsal horn ipsilateral to the injury. The neurons were multipolar and the reaction product revealed the morphology of the primary and secondary dendrites and some axons. Most of the neurons were located in the reticulated region of the dorsal horn, corresponding to Rexed's lamina V. In 14-day neuropathy animals treated with the NMDA-receptor antagonist MK-801, the number of RL-29 cell profiles observed was significantly reduced. Double labeling experiments revealed that spinothalamic tract neurons did not contain RL-29. The results suggest that the neuropathic injury produces a long term, transynaptic change in a subpopulation of dorsal horn neurons, that is mediated by excitatory amino acid transmitters acting at NMDA receptors.

    Original languageEnglish (US)
    Pages (from-to)64-71
    Number of pages8
    JournalBrain Research
    Volume620
    Issue number1
    DOIs
    StatePublished - Aug 20 1993

    Fingerprint

    Posterior Horn Cells
    Peripheral Nervous System Diseases
    Lectins
    Neurons
    N-Methyl-D-Aspartate Receptors
    Spinothalamic Tracts
    Excitatory Amino Acids
    Dizocilpine Maleate
    Wounds and Injuries
    Lactose
    Dendrites
    Axons
    Spinal Cord

    Keywords

    • Immediate early gene
    • NMDA antagonist
    • Sciatic nerve
    • Transynaptic induction

    ASJC Scopus subject areas

    • Developmental Biology
    • Molecular Biology
    • Clinical Neurology
    • Neuroscience(all)

    Cite this

    The endogenous lectin RL-29 is transynaptically induced in dorsal horn neurons following peripheral neuropathy in the rat. / Cameron, Adrian A.; Dougherty, Patrick M.; Garrison, Christopher J.; Willis, William D.; Carlton, Susan M.

    In: Brain Research, Vol. 620, No. 1, 20.08.1993, p. 64-71.

    Research output: Contribution to journalArticle

    Cameron, Adrian A. ; Dougherty, Patrick M. ; Garrison, Christopher J. ; Willis, William D. ; Carlton, Susan M. / The endogenous lectin RL-29 is transynaptically induced in dorsal horn neurons following peripheral neuropathy in the rat. In: Brain Research. 1993 ; Vol. 620, No. 1. pp. 64-71.
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    AU - Carlton, Susan M.

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