The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems

Marcie H. Moehnke, Terumi Midoro-Horiuti, Randall M. Goldblum, Christopher M. Kearney

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 μg/g leaf material) and Pichia pastoris (100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.

Original languageEnglish (US)
Pages (from-to)1259-1264
Number of pages6
JournalBiotechnology Letters
Volume30
Issue number7
DOIs
StatePublished - Jul 2008

Fingerprint

Allergens
Recombinant proteins
Recombinant Proteins
Yeast
Yeasts
Allergies
Proteins
Seasonal Allergic Rhinitis
Pichia
Extracellular Fluid
Pollen
Antibodies
Immunoglobulin E
Tobacco
Hypersensitivity
Fluids
Serum

Keywords

  • Jun a 3
  • Juniperus ashei
  • Pichia pastoris
  • Recombinant allergen
  • Tobacco mosaic virus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems. / Moehnke, Marcie H.; Midoro-Horiuti, Terumi; Goldblum, Randall M.; Kearney, Christopher M.

In: Biotechnology Letters, Vol. 30, No. 7, 07.2008, p. 1259-1264.

Research output: Contribution to journalArticle

Moehnke, Marcie H. ; Midoro-Horiuti, Terumi ; Goldblum, Randall M. ; Kearney, Christopher M. / The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems. In: Biotechnology Letters. 2008 ; Vol. 30, No. 7. pp. 1259-1264.
@article{49637e2ff0a445bc86812ba9bda7c9a7,
title = "The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems",
abstract = "Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 μg/g leaf material) and Pichia pastoris (100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.",
keywords = "Jun a 3, Juniperus ashei, Pichia pastoris, Recombinant allergen, Tobacco mosaic virus",
author = "Moehnke, {Marcie H.} and Terumi Midoro-Horiuti and Goldblum, {Randall M.} and Kearney, {Christopher M.}",
year = "2008",
month = "7",
doi = "10.1007/s10529-008-9665-x",
language = "English (US)",
volume = "30",
pages = "1259--1264",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "7",

}

TY - JOUR

T1 - The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems

AU - Moehnke, Marcie H.

AU - Midoro-Horiuti, Terumi

AU - Goldblum, Randall M.

AU - Kearney, Christopher M.

PY - 2008/7

Y1 - 2008/7

N2 - Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 μg/g leaf material) and Pichia pastoris (100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.

AB - Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 μg/g leaf material) and Pichia pastoris (100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.

KW - Jun a 3

KW - Juniperus ashei

KW - Pichia pastoris

KW - Recombinant allergen

KW - Tobacco mosaic virus

UR - http://www.scopus.com/inward/record.url?scp=44949251815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949251815&partnerID=8YFLogxK

U2 - 10.1007/s10529-008-9665-x

DO - 10.1007/s10529-008-9665-x

M3 - Article

VL - 30

SP - 1259

EP - 1264

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 7

ER -