TY - JOUR
T1 - The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems
AU - Moehnke, Marcie H.
AU - Midoro-Horiuti, Terumi
AU - Goldblum, Randall M.
AU - Kearney, Christopher M.
N1 - Funding Information:
Acknowledgments This work was supported by R01 AI052428 (R.M.G.) and K08 AI055792 (T.M.H.) from NIAID, and NIEHS Center for Environmental Science (#E06676, R.M.G.).
PY - 2008/7
Y1 - 2008/7
N2 - Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 μg/g leaf material) and Pichia pastoris (100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.
AB - Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed in Nicotiana benthamiana interstitial fluid (300 μg/g leaf material) and Pichia pastoris (100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.
KW - Jun a 3
KW - Juniperus ashei
KW - Pichia pastoris
KW - Recombinant allergen
KW - Tobacco mosaic virus
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U2 - 10.1007/s10529-008-9665-x
DO - 10.1007/s10529-008-9665-x
M3 - Article
C2 - 18270658
AN - SCOPUS:44949251815
SN - 0141-5492
VL - 30
SP - 1259
EP - 1264
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 7
ER -