The expression of antioxidant enzymes in a mouse model of fetal alcohol syndrome

Nathan Drever, Huaizhi Yin, Talar Kechichian, Maged Costantine, Monica Longo, George R. Saade, Egle Bytautiene

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

OBJECTIVE: Superoxide dismutase, glutathione peroxidase, and catalase prevent cellular damage produced by free radicals. Our objective was to evaluate if prenatal alcohol exposure decreased the expression of antioxidant enzymes in the brain, liver, or placenta of fetal mice. STUDY DESIGN: Timed, pregnant C57BL6/J mice were treated on gestational day 8 with intraperitoneal injection of alcohol (0.03 mL/g) or saline (control). Fetuses were harvested on gestational day 18. Fetal brain, liver, and placenta were analyzed for mRNA expression of superoxide dismutase, glutathione peroxidase, and catalase by real-time polymerase chain reaction, with 18S RNA used as reference. RESULTS: Superoxide dismutase, glutathione peroxidase, and catalase expression was lower in fetal brains exposed to alcohol with no differences detected in the liver or placenta between the 2 groups. CONCLUSION: Maternal alcohol consumption causes a decrease in superoxide dismutase, glutathione peroxidase, and catalase expression in the fetal brain. This may explain the long-term neurologic findings in fetal alcohol syndrome.

Original languageEnglish (US)
Pages (from-to)358.e19-358.e22
JournalAmerican journal of obstetrics and gynecology
Volume206
Issue number4
DOIs
StatePublished - Apr 2012

Keywords

  • antioxidant enzymes
  • fetal alcohol syndrome
  • fetus
  • mice

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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