The expression of antioxidant enzymes in a mouse model of fetal alcohol syndrome

Nathan Drever, Huaizhi Yin, Talar Kechichian, Maged Costantine, Monica Longo, George Saade, Egle Bytautiene

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE: Superoxide dismutase, glutathione peroxidase, and catalase prevent cellular damage produced by free radicals. Our objective was to evaluate if prenatal alcohol exposure decreased the expression of antioxidant enzymes in the brain, liver, or placenta of fetal mice. STUDY DESIGN: Timed, pregnant C57BL6/J mice were treated on gestational day 8 with intraperitoneal injection of alcohol (0.03 mL/g) or saline (control). Fetuses were harvested on gestational day 18. Fetal brain, liver, and placenta were analyzed for mRNA expression of superoxide dismutase, glutathione peroxidase, and catalase by real-time polymerase chain reaction, with 18S RNA used as reference. RESULTS: Superoxide dismutase, glutathione peroxidase, and catalase expression was lower in fetal brains exposed to alcohol with no differences detected in the liver or placenta between the 2 groups. CONCLUSION: Maternal alcohol consumption causes a decrease in superoxide dismutase, glutathione peroxidase, and catalase expression in the fetal brain. This may explain the long-term neurologic findings in fetal alcohol syndrome.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume206
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

Fetal Alcohol Spectrum Disorders
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Antioxidants
Placenta
Alcohols
Brain
Enzymes
Liver
Neurologic Manifestations
Intraperitoneal Injections
Alcohol Drinking
Free Radicals
Real-Time Polymerase Chain Reaction
Fetus
Mothers
RNA
Messenger RNA

Keywords

  • antioxidant enzymes
  • fetal alcohol syndrome
  • fetus
  • mice

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

The expression of antioxidant enzymes in a mouse model of fetal alcohol syndrome. / Drever, Nathan; Yin, Huaizhi; Kechichian, Talar; Costantine, Maged; Longo, Monica; Saade, George; Bytautiene, Egle.

In: American Journal of Obstetrics and Gynecology, Vol. 206, No. 4, 04.2012.

Research output: Contribution to journalArticle

Drever, Nathan ; Yin, Huaizhi ; Kechichian, Talar ; Costantine, Maged ; Longo, Monica ; Saade, George ; Bytautiene, Egle. / The expression of antioxidant enzymes in a mouse model of fetal alcohol syndrome. In: American Journal of Obstetrics and Gynecology. 2012 ; Vol. 206, No. 4.
@article{0ea2a7a6393a4684bee48299b219c002,
title = "The expression of antioxidant enzymes in a mouse model of fetal alcohol syndrome",
abstract = "OBJECTIVE: Superoxide dismutase, glutathione peroxidase, and catalase prevent cellular damage produced by free radicals. Our objective was to evaluate if prenatal alcohol exposure decreased the expression of antioxidant enzymes in the brain, liver, or placenta of fetal mice. STUDY DESIGN: Timed, pregnant C57BL6/J mice were treated on gestational day 8 with intraperitoneal injection of alcohol (0.03 mL/g) or saline (control). Fetuses were harvested on gestational day 18. Fetal brain, liver, and placenta were analyzed for mRNA expression of superoxide dismutase, glutathione peroxidase, and catalase by real-time polymerase chain reaction, with 18S RNA used as reference. RESULTS: Superoxide dismutase, glutathione peroxidase, and catalase expression was lower in fetal brains exposed to alcohol with no differences detected in the liver or placenta between the 2 groups. CONCLUSION: Maternal alcohol consumption causes a decrease in superoxide dismutase, glutathione peroxidase, and catalase expression in the fetal brain. This may explain the long-term neurologic findings in fetal alcohol syndrome.",
keywords = "antioxidant enzymes, fetal alcohol syndrome, fetus, mice",
author = "Nathan Drever and Huaizhi Yin and Talar Kechichian and Maged Costantine and Monica Longo and George Saade and Egle Bytautiene",
year = "2012",
month = "4",
doi = "10.1016/j.ajog.2012.01.017",
language = "English (US)",
volume = "206",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - The expression of antioxidant enzymes in a mouse model of fetal alcohol syndrome

AU - Drever, Nathan

AU - Yin, Huaizhi

AU - Kechichian, Talar

AU - Costantine, Maged

AU - Longo, Monica

AU - Saade, George

AU - Bytautiene, Egle

PY - 2012/4

Y1 - 2012/4

N2 - OBJECTIVE: Superoxide dismutase, glutathione peroxidase, and catalase prevent cellular damage produced by free radicals. Our objective was to evaluate if prenatal alcohol exposure decreased the expression of antioxidant enzymes in the brain, liver, or placenta of fetal mice. STUDY DESIGN: Timed, pregnant C57BL6/J mice were treated on gestational day 8 with intraperitoneal injection of alcohol (0.03 mL/g) or saline (control). Fetuses were harvested on gestational day 18. Fetal brain, liver, and placenta were analyzed for mRNA expression of superoxide dismutase, glutathione peroxidase, and catalase by real-time polymerase chain reaction, with 18S RNA used as reference. RESULTS: Superoxide dismutase, glutathione peroxidase, and catalase expression was lower in fetal brains exposed to alcohol with no differences detected in the liver or placenta between the 2 groups. CONCLUSION: Maternal alcohol consumption causes a decrease in superoxide dismutase, glutathione peroxidase, and catalase expression in the fetal brain. This may explain the long-term neurologic findings in fetal alcohol syndrome.

AB - OBJECTIVE: Superoxide dismutase, glutathione peroxidase, and catalase prevent cellular damage produced by free radicals. Our objective was to evaluate if prenatal alcohol exposure decreased the expression of antioxidant enzymes in the brain, liver, or placenta of fetal mice. STUDY DESIGN: Timed, pregnant C57BL6/J mice were treated on gestational day 8 with intraperitoneal injection of alcohol (0.03 mL/g) or saline (control). Fetuses were harvested on gestational day 18. Fetal brain, liver, and placenta were analyzed for mRNA expression of superoxide dismutase, glutathione peroxidase, and catalase by real-time polymerase chain reaction, with 18S RNA used as reference. RESULTS: Superoxide dismutase, glutathione peroxidase, and catalase expression was lower in fetal brains exposed to alcohol with no differences detected in the liver or placenta between the 2 groups. CONCLUSION: Maternal alcohol consumption causes a decrease in superoxide dismutase, glutathione peroxidase, and catalase expression in the fetal brain. This may explain the long-term neurologic findings in fetal alcohol syndrome.

KW - antioxidant enzymes

KW - fetal alcohol syndrome

KW - fetus

KW - mice

UR - http://www.scopus.com/inward/record.url?scp=84859430764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859430764&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2012.01.017

DO - 10.1016/j.ajog.2012.01.017

M3 - Article

C2 - 22365038

AN - SCOPUS:84859430764

VL - 206

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 4

ER -