The Flow Phase of Wound Metabolism Is Characterized by Stimulated Protein Synthesis Rather Than Cell Proliferation

Xiao jun Zhang, David L. Chinkes, Robert A. Cox, Robert R. Wolfe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Healing of a skin wound requires net protein deposition to repair the tissue defect and new epidermal cells to cover the wound. However, the true course of changes in rates of cell proliferation and protein deposition following skin injury has not been previously determined. This experiment was to measure DNA fractional synthetic rate (FSR), reflecting cell division, and protein FSR and fractional breakdown rate (FBR) in skin wound at three times after injury. Materials and methods: The experiment consisted of a surgery and a tracer infusion on separate days. During the surgery (day 0), a donor wound was created and indwelling catheters were inserted into the carotid artery and jugular vein under general anesthesia. On day 1, day 3, or day 7, stable isotope tracer infusion was performed in conscious rabbits to determine DNA FSR and protein FSR and FBR in the wound. Results: Protein FSR and FBR in the day 7 wound were 20.5 ± 8.4 and 12.6 ± 4.7%/day, respectively, which were greater (P < 0.01-0.05) than the corresponding values in the day 1 and day 3 wounds. Net protein deposition (FSR-FBR) in day 7 wound (7.9 ± 6.0%/day) was greater (P < 0.05) than in day 3 wound (0.8 ± 2.4%/day). DNA FSRs were 1.94 ± 0.58, 2.43 ± 0.96, 2.86 ± 0.90%/day in the day 1, day 3 and day 7 wounds, respectively (P = 0.2). Conclusions: The flow phase in the wound is characterized by increased protein synthesis rather than cell proliferation; net protein deposition in the wound is a major cause of protein requirements in severe burns.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalJournal of Surgical Research
Volume135
Issue number1
DOIs
StatePublished - Sep 2006

Fingerprint

Cell Proliferation
Wounds and Injuries
Proteins
Skin
DNA
Indwelling Catheters
Jugular Veins
Ambulatory Surgical Procedures
Burns
Carotid Arteries
Isotopes
Cell Division
General Anesthesia
Rabbits

Keywords

  • DNA synthesis
  • fractional breakdown rate
  • fractional synthetic rate
  • mass spectrometry
  • rabbits
  • stable isotopes
  • wound healing

ASJC Scopus subject areas

  • Surgery

Cite this

The Flow Phase of Wound Metabolism Is Characterized by Stimulated Protein Synthesis Rather Than Cell Proliferation. / Zhang, Xiao jun; Chinkes, David L.; Cox, Robert A.; Wolfe, Robert R.

In: Journal of Surgical Research, Vol. 135, No. 1, 09.2006, p. 61-67.

Research output: Contribution to journalArticle

Zhang, Xiao jun ; Chinkes, David L. ; Cox, Robert A. ; Wolfe, Robert R. / The Flow Phase of Wound Metabolism Is Characterized by Stimulated Protein Synthesis Rather Than Cell Proliferation. In: Journal of Surgical Research. 2006 ; Vol. 135, No. 1. pp. 61-67.
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title = "The Flow Phase of Wound Metabolism Is Characterized by Stimulated Protein Synthesis Rather Than Cell Proliferation",
abstract = "Background: Healing of a skin wound requires net protein deposition to repair the tissue defect and new epidermal cells to cover the wound. However, the true course of changes in rates of cell proliferation and protein deposition following skin injury has not been previously determined. This experiment was to measure DNA fractional synthetic rate (FSR), reflecting cell division, and protein FSR and fractional breakdown rate (FBR) in skin wound at three times after injury. Materials and methods: The experiment consisted of a surgery and a tracer infusion on separate days. During the surgery (day 0), a donor wound was created and indwelling catheters were inserted into the carotid artery and jugular vein under general anesthesia. On day 1, day 3, or day 7, stable isotope tracer infusion was performed in conscious rabbits to determine DNA FSR and protein FSR and FBR in the wound. Results: Protein FSR and FBR in the day 7 wound were 20.5 ± 8.4 and 12.6 ± 4.7{\%}/day, respectively, which were greater (P < 0.01-0.05) than the corresponding values in the day 1 and day 3 wounds. Net protein deposition (FSR-FBR) in day 7 wound (7.9 ± 6.0{\%}/day) was greater (P < 0.05) than in day 3 wound (0.8 ± 2.4{\%}/day). DNA FSRs were 1.94 ± 0.58, 2.43 ± 0.96, 2.86 ± 0.90{\%}/day in the day 1, day 3 and day 7 wounds, respectively (P = 0.2). Conclusions: The flow phase in the wound is characterized by increased protein synthesis rather than cell proliferation; net protein deposition in the wound is a major cause of protein requirements in severe burns.",
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N2 - Background: Healing of a skin wound requires net protein deposition to repair the tissue defect and new epidermal cells to cover the wound. However, the true course of changes in rates of cell proliferation and protein deposition following skin injury has not been previously determined. This experiment was to measure DNA fractional synthetic rate (FSR), reflecting cell division, and protein FSR and fractional breakdown rate (FBR) in skin wound at three times after injury. Materials and methods: The experiment consisted of a surgery and a tracer infusion on separate days. During the surgery (day 0), a donor wound was created and indwelling catheters were inserted into the carotid artery and jugular vein under general anesthesia. On day 1, day 3, or day 7, stable isotope tracer infusion was performed in conscious rabbits to determine DNA FSR and protein FSR and FBR in the wound. Results: Protein FSR and FBR in the day 7 wound were 20.5 ± 8.4 and 12.6 ± 4.7%/day, respectively, which were greater (P < 0.01-0.05) than the corresponding values in the day 1 and day 3 wounds. Net protein deposition (FSR-FBR) in day 7 wound (7.9 ± 6.0%/day) was greater (P < 0.05) than in day 3 wound (0.8 ± 2.4%/day). DNA FSRs were 1.94 ± 0.58, 2.43 ± 0.96, 2.86 ± 0.90%/day in the day 1, day 3 and day 7 wounds, respectively (P = 0.2). Conclusions: The flow phase in the wound is characterized by increased protein synthesis rather than cell proliferation; net protein deposition in the wound is a major cause of protein requirements in severe burns.

AB - Background: Healing of a skin wound requires net protein deposition to repair the tissue defect and new epidermal cells to cover the wound. However, the true course of changes in rates of cell proliferation and protein deposition following skin injury has not been previously determined. This experiment was to measure DNA fractional synthetic rate (FSR), reflecting cell division, and protein FSR and fractional breakdown rate (FBR) in skin wound at three times after injury. Materials and methods: The experiment consisted of a surgery and a tracer infusion on separate days. During the surgery (day 0), a donor wound was created and indwelling catheters were inserted into the carotid artery and jugular vein under general anesthesia. On day 1, day 3, or day 7, stable isotope tracer infusion was performed in conscious rabbits to determine DNA FSR and protein FSR and FBR in the wound. Results: Protein FSR and FBR in the day 7 wound were 20.5 ± 8.4 and 12.6 ± 4.7%/day, respectively, which were greater (P < 0.01-0.05) than the corresponding values in the day 1 and day 3 wounds. Net protein deposition (FSR-FBR) in day 7 wound (7.9 ± 6.0%/day) was greater (P < 0.05) than in day 3 wound (0.8 ± 2.4%/day). DNA FSRs were 1.94 ± 0.58, 2.43 ± 0.96, 2.86 ± 0.90%/day in the day 1, day 3 and day 7 wounds, respectively (P = 0.2). Conclusions: The flow phase in the wound is characterized by increased protein synthesis rather than cell proliferation; net protein deposition in the wound is a major cause of protein requirements in severe burns.

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