The force-driven conformations of heparin studied with single molecule force microscopy

Piotr E. Marszalek, Andres Oberhauser, Hongbin Li, Julio M. Fernandez

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Using single molecule force spectroscopy we examine the response of heparin chains to mechanical stretching. We find that at forces below 200 pN heparin behaves as a simple entropic spring. At ∼200 pN heparin displays a large enthalpic elasticity, which is evident as a pronounced plateau in the force-extension relationship. We determine that this enthalpic elasticity is produced by sugar rings of heparin flipping to more energetic and more extended conformations. We estimate that in vivo, the forces which stretch heparin are comparable to the forces that trigger conformational transitions in our single molecule atomic force microscopy measurements. We hypothesize that these conformational transitions have biological significance in that they provide a mechanism to finely regulate the affinity of various ligands toward heparin, for example, in secretory granules undergoing exocytosis and during the mechanical interactions between cells and the extracellular matrix.

Original languageEnglish (US)
Pages (from-to)2696-2704
Number of pages9
JournalBiophysical Journal
Volume85
Issue number4
StatePublished - Oct 1 2003

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Atomic Force Microscopy
Heparin
Elasticity
Exocytosis
Secretory Vesicles
Cell Communication
Extracellular Matrix
Single Molecule Imaging
Ligands

ASJC Scopus subject areas

  • Biophysics

Cite this

The force-driven conformations of heparin studied with single molecule force microscopy. / Marszalek, Piotr E.; Oberhauser, Andres; Li, Hongbin; Fernandez, Julio M.

In: Biophysical Journal, Vol. 85, No. 4, 01.10.2003, p. 2696-2704.

Research output: Contribution to journalArticle

Marszalek, Piotr E. ; Oberhauser, Andres ; Li, Hongbin ; Fernandez, Julio M. / The force-driven conformations of heparin studied with single molecule force microscopy. In: Biophysical Journal. 2003 ; Vol. 85, No. 4. pp. 2696-2704.
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