The functional consequences of cross-talk between the vitamin D receptor and ERK signaling pathways are cell-specific

Ramesh Narayanan, Veronica A. Tovar Sepulveda, Miriam Falzon, Nancy L. Weigel

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The actions of the active metabolite of 1,25-(OH)2D3 (1,25-D) are mediated primarily by the vitamin D receptor (VDR), a member of the nuclear receptor family of ligand-activated transcription factors. Although their ligands cause transcriptional activation, many of the ligands also rapidly activate cellular signaling pathways through mechanisms that have not been fully elucidated. We find that 1,25-D causes a rapid, but sustained activation of ERK (extracellular signal-regulated kinase) in bone cell lines. However, the effect of ERK activation on VDR transcriptional activity was cell line-specific. Inhibition of ERK activation by the MEK inhibitor, U0126, stimulated VDR activity im MC3T3-E1 cells, but inhibited the activity in MG-63 cells as well as in HeLa cells. VDR is not a known target of ERK. We found that the ERK target responsible for reduced VDR activity in MC3T3-E1 cells is RXRα. MC3T3-E1 cells express lower levels of RXRβ and RXRγ than either HeLa or MG-63 cells. Although overexpression of RXRα in MC3T3-E1 cells increased VDR activity, U0126 further enhanced the activity. In contrast, overexpression of RXRγ stimulated VDR activity but abrogated the stimulation by U0126. Thus, although 1,25-D treatment activates ERK in many cell types, subsequently inducing changes independent of VDR, the effects of treatment with 1,25-D on the transcriptional activity of VDR are RXR isoform-specific. In cells in which RXRα is the VDR partner, the transcriptional activation of VDR by 1,25-D is attenuated by the concomitant activation of ERK. In cells utilizing RXRγ, ERK activation enhances VDR transcriptional activity.

Original languageEnglish (US)
Pages (from-to)47298-47310
Number of pages13
JournalJournal of Biological Chemistry
Volume279
Issue number45
DOIs
StatePublished - Nov 5 2004

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Calcitriol Receptors
Extracellular Signal-Regulated MAP Kinases
Chemical activation
Ligands
Transcriptional Activation
Cells
Cell signaling
Cell Line
Mitogen-Activated Protein Kinase Kinases
Cytoplasmic and Nuclear Receptors
Metabolites
Nuclear Family
HeLa Cells
Protein Isoforms
Bone
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry

Cite this

The functional consequences of cross-talk between the vitamin D receptor and ERK signaling pathways are cell-specific. / Narayanan, Ramesh; Tovar Sepulveda, Veronica A.; Falzon, Miriam; Weigel, Nancy L.

In: Journal of Biological Chemistry, Vol. 279, No. 45, 05.11.2004, p. 47298-47310.

Research output: Contribution to journalArticle

Narayanan, Ramesh ; Tovar Sepulveda, Veronica A. ; Falzon, Miriam ; Weigel, Nancy L. / The functional consequences of cross-talk between the vitamin D receptor and ERK signaling pathways are cell-specific. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 45. pp. 47298-47310.
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