The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies

K. Mavromatis, C. Kuyler Doyle, A. Lykidis, N. Ivanova, M. P. Francino, P. Chain, M. Shin, S. Malfatti, F. Larimer, A. Copeland, J. C. Detter, M. Land, P. M. Richardson, X. J. Yu, David Walker, Jere McBride, N. C. Kyrpides

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Ehrlichia canis, a small obligately intracellular, tick-transmitted, gram-negative, α-proteobacterium, is the primary etiologic agent of globally distributed canine monocytic ehrlichiosis. Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome of 1,315,030 bp predicted to encode 925 proteins, 40 stable RNA species, 17 putative pseudogenes, and a substantial proportion of noncoding sequence (27%). Interesting genome features include a large set of proteins with transmembrane helices and/or signal sequences and a unique serine-threonine bias associated with the potential for O glycosylation that was prominent in proteins associated with pathogen-host interactions. Furthermore, two paralogous protein families associated with immune evasion were identified, one of which contains poly(G-C) tracts, suggesting that they may play a role in phase variation and facilitation of persistent infections. Genes associated with pathogen-host interactions were identified, including a small group encoding proteins (n = 12) with tandem repeats and another group encoding proteins with eukaryote-like ankyrin domains (n = 7).

Original languageEnglish (US)
Pages (from-to)4015-4023
Number of pages9
JournalJournal of Bacteriology
Volume188
Issue number11
DOIs
StatePublished - Jun 2006

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Ehrlichia canis
Immune Evasion
Genome
Bacteria
Membranes
Host-Pathogen Interactions
Proteins
Ehrlichiosis
Ankyrins
Proteobacteria
Pseudogenes
Tandem Repeat Sequences
Chromosomes, Human, Pair 1
Ticks
Threonine
Protein Sorting Signals
Eukaryota
Glycosylation
Serine
Canidae

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Mavromatis, K., Doyle, C. K., Lykidis, A., Ivanova, N., Francino, M. P., Chain, P., ... Kyrpides, N. C. (2006). The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies. Journal of Bacteriology, 188(11), 4015-4023. https://doi.org/10.1128/JB.01837-05

The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies. / Mavromatis, K.; Doyle, C. Kuyler; Lykidis, A.; Ivanova, N.; Francino, M. P.; Chain, P.; Shin, M.; Malfatti, S.; Larimer, F.; Copeland, A.; Detter, J. C.; Land, M.; Richardson, P. M.; Yu, X. J.; Walker, David; McBride, Jere; Kyrpides, N. C.

In: Journal of Bacteriology, Vol. 188, No. 11, 06.2006, p. 4015-4023.

Research output: Contribution to journalArticle

Mavromatis, K, Doyle, CK, Lykidis, A, Ivanova, N, Francino, MP, Chain, P, Shin, M, Malfatti, S, Larimer, F, Copeland, A, Detter, JC, Land, M, Richardson, PM, Yu, XJ, Walker, D, McBride, J & Kyrpides, NC 2006, 'The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies', Journal of Bacteriology, vol. 188, no. 11, pp. 4015-4023. https://doi.org/10.1128/JB.01837-05
Mavromatis, K. ; Doyle, C. Kuyler ; Lykidis, A. ; Ivanova, N. ; Francino, M. P. ; Chain, P. ; Shin, M. ; Malfatti, S. ; Larimer, F. ; Copeland, A. ; Detter, J. C. ; Land, M. ; Richardson, P. M. ; Yu, X. J. ; Walker, David ; McBride, Jere ; Kyrpides, N. C. / The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 11. pp. 4015-4023.
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AU - Richardson, P. M.

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